Pesticide tolerance induced by a generalized stress response in wood frogs (Rana sylvatica)
Pesticide tolerance induced by a generalized stress response in wood frogs (Rana sylvatica)
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林蛙（Rana sylvatica）普遍应激反应诱导的农药耐受性
DOI：
10.1007/s10646-020-02277-2
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发表时间：
2020
期刊：
影响因子：
2.7
通讯作者：
Hoverman, Jason T.
中科院分区：
文献类型：
--
作者：
Billet, Logan S.;Hoverman, Jason T.
越来越多的证据表明，表型可塑性在生态毒理学中起着至关重要的作用。更具体地说，诱导农药耐受性，其中暴露于污染物的人群显示出对污染物的耐受性增加，已经在多个分类群中记录。然而，诱导耐受的生理机制仍不清楚。我们假设，诱导农药耐受性是一个广义的压力反应的结果，根据以往的研究表明，自然压力和人为压力可以诱导耐受农药。我们测试了这一假设，首先暴露幼虫林蛙（林蛙sylvatica）的人为应激源（亚致死西维因浓度），一个自然的应激源（线索从笼中捕食者），或模拟应激源通过外源性暴露于应激激素皮质酮（125 nM）。我们还纳入了用化合物甲吡酮（MTP）抑制皮质酮合成的治疗。然后，我们将幼虫暴露于致命的西维因治疗，以评估死亡时间。我们发现，事先暴露于125 nM的外源性CORT和捕食者线索诱导耐受致死浓度的西维因通过轻微的延迟时间死亡。预暴露于亚致死西维因，以及MTP单独或与捕食者线索相结合，没有诱导耐受性的致死西维因浓度相对于乙醇车辆控制处理。我们的研究提供的证据表明，农药耐受性可以诱导的一个广义的压力反应的存在和不存在（外源CORT）的特定线索，并强调了考虑生理生态和环境背景下的生态毒理学的重要性。
Increasing evidence suggests that phenotypic plasticity can play a critical role in ecotoxicology. More specifically, induced pesticide tolerance, in which populations exposed to a contaminant show increased tolerance to the contaminants later, has been documented in multiple taxa. However, the physiological mechanisms of induced tolerance remain unclear. We hypothesized that induced pesticide tolerance is the result of a generalized stress response based on previous studies showing that both natural stressors and anthropogenic stressors can induce tolerance to pesticides. We tested this hypothesis by first exposing larval wood frogs (Rana sylvatica) to either an anthropogenic stressor (sublethal carbaryl concentration), a natural stressor (cues from a caged predator), or a simulated stressor via exogenous exposure to the stress hormone corticosterone (125 nM). We also included treatments that inhibited corticosterone synthesis with the compound metyrapone (MTP). We then exposed the larvae to a lethal carbaryl treatment to assess time to death. We found that prior exposure to 125 nM of exogenous CORT and predator cues induced tolerance to a lethal concentration of carbaryl through a slight delay in time to death. Pre-exposure to sublethal carbaryl, as well as MTP alone or in combination with predator cues, did not induce tolerance to the lethal carbaryl concentration relative to the ethanol vehicle control treatment. Our study provides evidence that pesticide tolerance can be induced by a generalized stress response both in the presence and absence (exogenous CORT) of specific cues and highlights the importance of considering physiological ecology and environmental context in ecotoxicology.
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影响因子：
13.6
作者：
Haddad NM;Brudvig LA;Clobert J;Davies KF;Gonzalez A;Holt RD;Lovejoy TE;Sexton JO;Austin MP;Collins CD;Cook WM;Damschen EI;Ewers RM;Foster BL;Jenkins CN;King AJ;Laurance WF;Levey DJ;Margules CR;Melbourne BA;Nicholls AO;Orrock JL;Song DX;Townshend JR
通讯作者：
Townshend JR
捕食者引起的压力使杀虫剂西维因对灰树蛙蝌蚪（Hyla versicolor）更加致命
DOI：
--
发表时间：
2001
影响因子：
11.1
作者：
R. Relyea;Nathan Mills
通讯作者：
Nathan Mills
DOI：
10.2307/2419653
发表时间：
1998-05
期刊：
--
影响因子：
--
作者：
C. Schlichting;M. Pigliucci
通讯作者：
C. Schlichting;M. Pigliucci
影响因子：
2.7
作者：
O'Connor, Constance M.;Norris, D. Ryan;Cooke, Steven J.
通讯作者：
Cooke, Steven J.
影响因子：
2.1
作者：
Tang, Yuan;Horikoshi, Masaaki;Li, Wenxuan
通讯作者：
Li, Wenxuan