Sterols from Mytilidae show anti-aging and neuroprotective effects via anti-oxidative activity.
Sterols from Mytilidae show anti-aging and neuroprotective effects via anti-oxidative activity.
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DOI：
10.3390/ijms151221660
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发表时间：
2014-11-25
影响因子：
5.6
通讯作者：
Qi J
中科院分区：
文献类型：
--
作者：
Sun Y;Lin Y;Cao X;Xiang L;Qi J
为了从海洋天然产物中筛选抗衰老样品，采用K6001酵母菌株作为生物测定系统。分离活性贻贝提取物以得到活性甾醇级分（SF）。 SF进一步纯化，得到四种甾醇化合物。它们的结构被确定为胆固醇 (CHOL)、菜子甾醇、crinosterol 和 24-亚甲基胆固醇。所有化合物均表现出相似的抗衰老活性。为了了解所涉及的作用机制，对最丰富的化合物 CHOL 进行了抗氧化实验、活性氧 (ROS) 测定和丙二醛 (MDA) 测试。结果表明，CHOL 处理提高了酵母在氧化应激下的存活率，并降低了 ROS 和 MDA 水平。此外，还采用了具有K6001背景的uth1、skn7、sod1和sod2突变，并且突变的寿命不受CHOL的影响。这些结果表明 CHOL 通过抗氧化应激发挥抗衰老作用。基于神经保护与抗衰老之间的联系，在PC12细胞中进行了神经保护实验。使用百草枯诱导氧化应激，结果表明CHOL和SF可以保护PC12细胞免受百草枯诱导的损伤。此外，这些物质表现出的神经生长因子（NGF）模拟活性再次证实了它们的神经保护功能。
For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to be cholesterol (CHOL), brassicasterol, crinosterol, and 24-methylenecholesterol. All compounds showed similar anti-aging activity. To understand the action mechanism involved, anti-oxidative experiments, reactive oxygen species (ROS) assays, and malondialdehyde (MDA) tests were performed on the most abundant compound, CHOL. Results indicated that treatment with CHOL increases the survival rate of yeast under oxidative stress and decreases ROS and MDA levels. In addition, mutations of uth1, skn7, sod1, and sod2, which feature a K6001 background, were employed and the lifespans of the mutations were not affected by CHOL. These results demonstrate that CHOL exerts anti-aging effects via anti-oxidative stress. Based on the connection between neuroprotection and anti-aging, neuroprotective experiments were performed in PC12 cells. Paraquat was used to induce oxidative stress and the results showed that the CHOL and SF protect the PC12 cells from the injury induced by paraquat. In addition, these substance exhibited nerve growth factor (NGF) mimic activities again confirmed their neuroprotective function.
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影响因子：
3.6
作者：
BERGMANN, W;FEENEY, RJ
通讯作者：
FEENEY, RJ
影响因子：
6.1
作者：
Wu, Wanqiang;Wang, Xin;Liu, Xuebo
通讯作者：
Liu, Xuebo
影响因子：
11.4
作者：
Kim, Dohoon;Nguyen, Minh Dang;Tsai, Li-Huei
通讯作者：
Tsai, Li-Huei
影响因子：
14
作者：
Thies, William;Bleiler, Laura
通讯作者：
Bleiler, Laura
影响因子：
--
作者：
Mulford, K. E.;Fassler, J. S.
通讯作者：
Fassler, J. S.