Monitoring Visual Cortical Activities During Progressive Retinal Degeneration Using Functional Bioluminescence Imaging.
Monitoring Visual Cortical Activities During Progressive Retinal Degeneration Using Functional Bioluminescence Imaging.
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DOI：
10.3389/fnins.2021.750684
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发表时间：
2021
影响因子：
4.3
通讯作者：
Mohanty SK
中科院分区：
文献类型：
--
作者：
Narcisse D;Mustafi SM;Carlson M;Kim S;Batabyal S;Wright W;Mohanty SK
遗传性视网膜退行性疾病（例如色素性视网膜炎）的小鼠模型的特征是光感受器变性，这阻碍了传输到视觉皮层的信号的产生。通过监测 Ca2+ 生物发光神经活动，我们量化了 RD10 小鼠在视网膜变性过程中视觉皮层活动响应视觉刺激的变化，这与眼睛视网膜电图信号的逐渐恶化相关。视觉皮层中活跃神经元的数量、Ca2+生物发光反应的强度和神经激活参数在衰老过程中表现出逐渐恶化。此外，我们将光学相干断层扫描测量的视网膜变薄与视网膜变性进展时视觉皮层活动的减少联系起来。本研究将 Ca2+-生物发光监测建立为一种纵向成像方式，用于表征视网膜退行性疾病模型视觉皮层的活动和治疗干预。
Mouse models of inherited retinal degenerative diseases such as retinitis pigmentosa are characterized by degeneration of photoreceptors, which hinders the generation of signal to be transmitted to the visual cortex. By monitoring Ca2+-bioluminescence neural activity, we quantified changes in visual cortical activities in response to visual stimuli in RD10 mice during progression of retinal degeneration, which correlated with progressive deteriorations of electro-retinography signal from the eyes. The number of active neurons in the visual cortex, the intensity of Ca2+-bioluminescence response, and neural activation parameter showed progressive deterioration during aging. Further, we correlated the thinning of retina as measured by Optical Coherence Tomography with the decrease in visual cortical activities as retinal degeneration progressed. The present study establishes Ca2+-bioluminescence monitoring as a longitudinal imaging modality to characterize activities in visual cortex of retinal degenerative disease models and therapeutic interventions.
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影响因子：
4.4
作者：
Huber G;Beck SC;Grimm C;Sahaboglu-Tekgoz A;Paquet-Durand F;Wenzel A;Humphries P;Redmond TM;Seeliger MW;Fischer MD
通讯作者：
Fischer MD
影响因子：
4.3
作者：
Prodanov D;Delbeke J
通讯作者：
Delbeke J
DOI：
10.1002/cne.22322
发表时间：
2010-06-01
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Phillips MJ;Otteson DC;Sherry DM
通讯作者：
Sherry DM
影响因子：
3.7
作者：
Oh J;Kim SH;Kim YJ;Lee H;Cho JH;Cho YH;Kim CK;Lee TJ;Lee S;Park KH;Yu HG;Lee HJ;Jun SC;Kim JH
通讯作者：
Kim JH
影响因子：
0.8
作者：
Benchorin, Gillie;Calton, Melissa A.;Vollrath, Douglas
通讯作者：
Vollrath, Douglas