Integration of Self-Assembled Microvascular Networks with Microfabricated PEG-Based Hydrogels.
Integration of Self-Assembled Microvascular Networks with Microfabricated PEG-Based Hydrogels.
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DOI：
10.1002/adfm.201200976
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发表时间：
2012-11-07
影响因子：
19
通讯作者：
West, Jennifer L.
中科院分区：
文献类型：
--
作者：
Cuchiara, Michael P.;Gould, Daniel J.;McHale, Melissa K.;Dickinson, Mary E.;West, Jennifer L.
尽管付出了巨大的努力，但组织工程构建物仅限于仅通过扩散维持的薄的简单组织。组织工程中最重要的障碍是血管化不足，无法在体外发育过程中输送营养物质和代谢物，也无法促进体内快速血管整合。组织工程构建体可以通过在体外开发可灌注的微血管网络以提供模拟天然血管组织和功能的运输来大大改进。在这里，微流体水凝胶与自组装促血管生成共培养物在体外灌注微血管网络的策略中整合。该方法允许控制微血管网络自组装，并采用吻合界面将自组装的微血管网络与制造的微通道整合。因此，系统内的传输从简单扩散转变为容器支持的对流传输和容器外扩散，从而改善整体质量传输特性。这项工作影响了体外可灌注预血管化组织的发展，并最终影响了体内组织工程的应用。
Despite tremendous efforts, tissue engineered constructs are restricted to thin, simple tissues sustained only by diffusion. The most significant barrier in tissue engineering is insufficient vascularization to deliver nutrients and metabolites during development in vitro and to facilitate rapid vascular integration in vivo. Tissue engineered constructs can be greatly improved by developing perfusable microvascular networks in vitro in order to provide transport that mimics native vascular organization and function. Here a microfluidic hydrogel is integrated with a self-assembling pro-vasculogenic co-culture in a strategy to perfuse microvascular networks in vitro. This approach allows for control over microvascular network self-assembly and employs an anastomotic interface for integration of self-assembled micro-vascular networks with fabricated microchannels. As a result, transport within the system shifts from simple diffusion to vessel supported convective transport and extra-vessel diffusion, thus improving overall mass transport properties. This work impacts the development of perfusable prevascularized tissues in vitro and ultimately tissue engineering applications in vivo.
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影响因子：
14
作者：
Cuchiara, Michael P.;Allen, Alicia C. B.;West, Jennifer L.
通讯作者：
West, Jennifer L.
影响因子：
6.1
作者：
Ling, Yibo;Rubin, Jamie;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
3.8
作者：
Malda, J;Rouwkema, J;Riesle, J
通讯作者：
Riesle, J
影响因子：
3.1
作者：
GERLOWSKI, LE;JAIN, RK
通讯作者：
JAIN, RK
影响因子：
--
作者：
Clark, ER;Clark, EL
通讯作者：
Clark, EL