A Conserved Basal Transcription Factor Is Required for the Function of Diverse TAL Effectors in Multiple Plant Hosts.
A Conserved Basal Transcription Factor Is Required for the Function of Diverse TAL Effectors in Multiple Plant Hosts.
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多种植物宿主中多种 TAL 效应子的功能需要保守的基础转录因子。
DOI：
10.3389/fpls.2017.01919
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发表时间：
2017
影响因子：
5.6
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Huang R;Hui S;Zhang M;Li P;Xiao J;Li X;Yuan M;Wang S
许多黄单胞菌利用转录激活因子样效应蛋白（transcription activator-like effector， TALE）激活植物疾病易感基因（disease susceptibility， S），这种激活有助于疾病的发生。我们最近报道了水稻基础转录因子IIAγ亚基ostfiia γ - 5被携带tale的水稻黄单胞菌侵染植株。然而，tfiia γ - s是否也参与其他植物中携带tale的黄单胞菌引起的疾病尚不清楚。本文采用分子和遗传学方法研究了TFIIAγs在其他植物中的作用。我们发现tfiia γ - s也被携带tale的黄单胞菌利用来引起其他植物的疾病。柑桔黄单胞菌的故事。柑橘（Xcc）引起柑橘和油菜黄单胞菌溃疡病。辣椒和番茄中引起细菌性斑疹的白斑病（Xcv）与相应的寄主tfiia - γ - s相互作用。通过转录抑制tfiiia γ导致柑橘抗Xcc，辣椒和番茄抗Xcv。ostfiia γ - 5和柑橘CsTFIIAγ的第39个残基对植物S基因的tale依赖诱导至关重要。突变的OsTFIIAγ5V 39E、CsTFIIAγV 39E、辣椒CaTFIIAγV 39E和番茄SlTFIIAγV 39E也不与TALEs相互作用。这些结果表明，携带tale的细菌具有感染植物的共同机制。利用tfiia γ - v 39e型突变可以作为提高作物对携带tale病原菌抗性的一般策略。
Many Xanthomonas bacteria use transcription activator-like effector (TALE) proteins to activate plant disease susceptibility (S) genes, and this activation contributes to disease. We recently reported that rice basal transcription factor IIA gamma subunit, OsTFIIAγ5, is hijacked by TALE-carrying Xanthomonas oryzae infecting the plants. However, whether TFIIAγs are also involved in TALE-carrying Xanthomonas-caused diseases in other plants is unknown. Here, molecular and genetic approaches were used to investigate the role of TFIIAγs in other plants. We found that TFIIAγs are also used by TALE-carrying Xanthomonas to cause disease in other plants. The TALEs of Xanthomonas citri pv. citri (Xcc) causing canker in citrus and Xanthomonas campestris pv. vesicatoria (Xcv) causing bacterial spot in pepper and tomato interacted with corresponding host TFIIAγs as in rice. Transcriptionally suppressing TFIIAγ led to resistance to Xcc in citrus and Xcv in pepper and tomato. The 39th residue of OsTFIIAγ5 and citrus CsTFIIAγ is vital for TALE-dependent induction of plant S genes. As mutated OsTFIIAγ5V 39E, CsTFIIAγV 39E, pepper CaTFIIAγV 39E, and tomato SlTFIIAγV 39E also did not interact with TALEs to prevent disease. These results suggest that TALE-carrying bacteria share a common mechanism for infecting plants. Using TFIIAγV 39E-type mutation could be a general strategy for improving resistance to TALE-carrying pathogens in crops.
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影响因子：
19
作者：
Doyle, Erin L.;Stoddard, Barry L.;Voytas, Daniel F.;Bogdanove, Adam J.
通讯作者：
Bogdanove, Adam J.
影响因子：
4.9
作者：
Ohyanagi H;Ebata T;Huang X;Gong H;Fujita M;Mochizuki T;Toyoda A;Fujiyama A;Kaminuma E;Nakamura Y;Feng Q;Wang ZX;Han B;Kurata N
通讯作者：
Kurata N
DOI：
10.1073/pnas.0701742104
发表时间：
2007-06-19
影响因子：
11.1
作者：
Sugio, Akiko;Yang, Bing;White, Frank F.
通讯作者：
White, Frank F.
影响因子：
6.2
作者：
Ge, XJ;Chu, ZH;Wang, SP
通讯作者：
Wang, SP
影响因子：
3.5
作者：
BONAS, U;SCHULTE, R;STALL, RE
通讯作者：
STALL, RE