Ascorbic acid specifically reduces the misclassification of nonirritating reactive chemicals in the OptiSafe™ macromolecular eye irritation test.
Ascorbic acid specifically reduces the misclassification of nonirritating reactive chemicals in the OptiSafe™ macromolecular eye irritation test.
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抗坏血酸特异性减少了OptISAFE™大分子眼刺激测试中非辐射反应性化学物质的错误分类。
DOI：
10.1016/j.tiv.2022.105313
复制
发表时间：
2022-04
影响因子：
3.2
通讯作者：
Jester, James, V
中科院分区：
文献类型：
--
作者：
Lebrun, Stewart;Chavez, Sara;Chan, Roxanne;Nguyen, Linda;Jester, James, V
关键词：
最近，我们发现在OptiSafe™大分子眼刺激试验中添加生理浓度的抗坏血酸（一种泪液抗氧化剂）可降低假阳性（FP）化学品的OptiSafe刺激评分，这些化学品具有导致活性氧（ROS）形成和分子交联的反应性化学物质。本研究的目的是：1）增加检测的化学品数量，以全面确定抗氧化剂相关的光密度（OD）降低是否特异于与ROS化学相关的FP化学品，2）确定添加抗氧化剂是否干扰真阳性（TP）和真阴性（TN）眼刺激物的检测。我们报告说，当抗坏血酸加入到测试试剂中时，用反应性化学品重新测试FP化学品显示OD值显著降低（P<0.05）。重要的是，抗坏血酸对TP或TN化学品的OD值没有显着影响，无论化学反应。这些结果表明，在替代性眼刺激试验中补充抗坏血酸可能有助于提高TN对那些经常被错误分类的反应性化学品的检测。
Recently, we showed that the addition of physiological concentrations of ascorbic acid, a tear antioxidant, to the OptiSafe™ macromolecular eye irritation test reduced the OptiSafe irritation scores of false-positive (FP) chemicals that had reactive chemistries leading to formation of reactive oxygen species (ROS) and molecular crosslinking. The purpose of the current study was to 1) increase the number of chemicals tested to comprehensibly determine whether the antioxidant-associated reduction in optical density (OD) is specific to FP chemicals associated with ROS chemistries, and 2) determine whether the addition of antioxidants interferes with the detection of true positive (TP) and true negative (TN) ocular irritants. We report that when ascorbic acid is added to the test reagents, retesting of FP chemicals with reactive chemistries show significantly reduced OD values (P<0.05). Importantly, ascorbic acid had no significant effect on the OD values of TP or TN chemicals regardless of chemical reactivity. These findings suggest that supplementation of ascorbic acid in alternative ocular irritation test may help improve the detection of TN for those commonly misclassified reactive chemicals.
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DOI：
10.1016/0165-7992(92)90145-8
发表时间：
1992-10-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
KUYKENDALL, JR;BOGDANFFY, MS
通讯作者：
BOGDANFFY, MS
影响因子：
3.2
作者：
Divakaran, Anumon V.;Torris, Arun A. T.;Badiger, Manohar V.
通讯作者：
Badiger, Manohar V.
影响因子：
10.8
作者：
Iza, M;Stoianovici, G;Couarraze, G
通讯作者：
Couarraze, G
影响因子：
15
作者：
BROWN, N;ANDERSON, AW;SCHWEITZER, CE
通讯作者：
SCHWEITZER, CE
影响因子：
5.8
作者：
Ben Amara, Ibtissem;Ben Saad, Hajer;Zeghal, Najiba
通讯作者：
Zeghal, Najiba