Beneficial effects of ascorbic acid to treat lung fibrosis induced by paraquat.
Beneficial effects of ascorbic acid to treat lung fibrosis induced by paraquat.
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DOI：
10.1371/journal.pone.0205535
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Lino-Dos-Santos-Franco A
中科院分区：
文献类型：
--
作者：
Rodrigues da Silva M;Schapochnik A;Peres Leal M;Esteves J;Bichels Hebeda C;Sandri S;Pavani C;Ratto Tempestini Horliana AC;Farsky SHP;Lino-Dos-Santos-Franco A
百草枯 (PQ) 是全世界使用最广泛的除草剂之一，对人类和动物造成严重的毒性作用。 PQ 暴露可导致肺纤维化 (PF)，其机制似乎与氧化应激有关，尽管也有人提出了其他途径。抗氧化剂可以作为一种有用的治疗方法，尽管对这种系统的干预仍然存在争议。因此，本研究研究了抗坏血酸（维生素 C）治疗后对雄性 C57/BL6 小鼠 PQ 诱导的 PF 的作用。通过单次 PQ 注射（10mg/kg；腹腔注射）诱导肺纤维化。对照组接受了 PQ 车辆。注射 PQ 或媒介物 7 天后，小鼠在接下来的 7 天内接受维生素 C（150 毫克/千克，腹膜内注射，每天一次）或媒介物。最后一次服用维生素 C 或媒介物后 24 小时，对小鼠实施安乐死，并收集其支气管肺泡灌洗液 (BALF) 和肺部。获得的数据表明，维生素 C 减少了细胞募集、IL-17（一种参与中性粒细胞迁移的细胞因子）、TGF-β（一种促纤维化介质）和胶原蛋白沉积的分泌。此外，维生素 C 可以升高超氧化物歧化酶 (SOD) 和过氧化氢酶（这两种抗氧化酶）的水平，但它不会改变乙酰甲胆碱引起的气管收缩反应。因此，研究人员强调维生素 C 的非侵入性机制，并建议将其作为治疗由百草枯中毒引起的肺纤维化的有前途的工具。
Paraquat (PQ) is one of the most widely employed herbicides that is used worldwide and it causes severe toxic effects in humans and animals. A PQ exposition can lead to pulmonary fibrosis (PF) and the mechanisms seem to be linked to oxidative stress, although other pathways have been suggested. Antioxidants can be useful as a therapy, although interventions with this kind of system are still controversial. Hence, this study has investigated the role of ascorbic acid (vitamin C) post-treatment on PQ-induced PF in male C57/BL6 mice. Pulmonary fibrosis was induced by a single PQ injection (10mg/kg; i.p.). The control group received a PQ vehicle. Seven days after the PQ or vehicle injections, the mice received vitamin C (150 mg/kg, ip, once a day) or the vehicle, over the following 7 days. Twenty-four hours after the last dose of vitamin C or the vehicle, the mice were euthanized and their bronchoalveolar lavage fluid (BALF) and their lungs were collected. The data obtained showed that vitamin C reduced the cellular recruitment, the secretion of IL-17 –a cytokine involved in neutrophils migration, TGF-β–a pro-fibrotic mediator and the collagen deposition. Moreover, vitamin C elevated the superoxide dismutase (SOD) and catalase levels, both antioxidant enzymes, but it did not alter the tracheal contractile response that was evoked by methacholine. Therefore, the researchers have highlighted the mechanisms of vitamin C as being non-invasive and have suggested it as a promising tool to treat lung fibrosis when it is induced by a PQ intoxication.
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影响因子：
24.3
作者：
Beeh, KM;Beier, J;Buhl, R
通讯作者：
Buhl, R
影响因子：
2.1
作者：
Brochetti, Robson Alexandre;Leal, Mayara Peres;Lino-dos-Santos-Franco, Adriana
通讯作者：
Lino-dos-Santos-Franco, Adriana
影响因子：
7.1
作者：
GANGULY, R;DURIEUX, MF;WALDMAN, RH
通讯作者：
WALDMAN, RH
DOI：
10.1152/ajplung.00300.2011
发表时间：
2012-07-01
影响因子：
4.9
作者：
Fisher, Bernard J.;Kraskauskas, Donatas;Natarajan, Ramesh
通讯作者：
Natarajan, Ramesh
MAPK 通路介导百草枯诱导的肺泡上皮细胞上皮间质转化
影响因子：
4.5
作者：
Huang, Min;Wang, Ya-Peng;Yang, Hui-Fang
通讯作者：
Yang, Hui-Fang