Establishing new sites of polarization by microtubules.
Establishing new sites of polarization by microtubules.
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DOI：
10.1016/j.cub.2008.12.008
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发表时间：
2009-01-27
期刊：
影响因子：
--
通讯作者：
Chang F
中科院分区：
文献类型：
--
作者：
Minc N;Bratman SV;Basu R;Chang F
微管（MT）参与肌动蛋白为基础的过程，如胞质分裂和细胞极化的空间调节。裂殖酵母裂殖酵母是一种杆状细胞，其在细胞尖端表现出极化细胞生长。MT+末端接触并从细胞尖端收缩，有助于极性调节。在这里，我们调查的影响，改变细胞形状的MT和细胞极化机制。我们用物理方法使分裂酵母细胞弯曲，迫使它们进入微型的飞升室。在这些弯曲的细胞中，MT保持直轴，并从细胞侧面的皮质部位接触和收缩。在这些异位位点，极性因子如bud 6p、for 3 p（cnt）和cdc 42 p被募集并以MT依赖性方式组装肌动蛋白电缆。MT在皮层的接触在几秒钟内诱导bud 6p点的出现。当MT相关的极性因子tea 1 p不存在时，极性因子的积累导致这些位点的细胞生长。这一过程依赖于MT、mal 3 p（EB 1）、moe 1 p（EB 1结合蛋白）和for 3 p，但令人惊讶的是，它不依赖于tea 1 p-tea 4p通路。这些研究提供了一个直接的示范如何MT诱导肌动蛋白组装在特定位置的细胞皮质和开始，以确定一个新的途径参与这一过程。MT与皮质的相互作用可能受到皮质附着位点的调节。这些发现突出了细胞形状、极性机制和负责细胞形态发生的MT之间的串扰。
Microtubules (MTs) participate in the spatial regulation of actin-based processes such as cytokinesis and cell polarization. The fission yeast Schizosaccharomyces pombe is a rod-shaped cell which exhibits polarized cell growth at cell tips. MT plus ends contact and shrink from the cell tips and contribute to polarity regulation. Here, we investigate the effects of changing cell shape on MTs and cell polarization machinery. We physically bend fission yeast cells by forcing them into microfabricated femtoliter chambers. In these bent cells, MTs maintain a straight axis and contact and shrink from cortical sites at the sides of cells. At these ectopic sites, polarity factors such as bud6p, for3p (formin), and cdc42p are recruited and assemble actin cables in an MT-dependent manner. MT contact at the cortex induces the appearance of a bud6p dot within seconds. The accumulation of polarity factors leads to cell growth at these sites, when the MT-associated polarity factor tea1p is absent. This process is dependent on MTs, mal3p (EB1), moe1p (an EB1-binding protein), and for3p, but surprisingly, is independent of the tea1p–tea4p pathway. These studies provide a direct demonstration for how MTs induce actin assembly at specific locations on the cell cortex and begin to identify a new pathway involved in this process. MT interactions with the cortex may be regulated by cortical attachment sites. These findings highlight the crosstalk between cell shape, polarity mechanisms, and MTs responsible for cell morphogenesis.
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DOI：
10.1083/jcb.200301147
发表时间：
2003-06-23
期刊：
The Journal of cell biology
影响因子：
--
作者：
Janson ME;de Dood ME;Dogterom M
通讯作者：
Dogterom M
影响因子：
10.5
作者：
Grallert, Agnes;Beuter, Christoph;Hagan, Iain M.
通讯作者：
Hagan, Iain M.
影响因子：
64.8
作者：
Keren, Kinneret;Pincus, Zachary;Theriot, Julie A.
通讯作者：
Theriot, Julie A.
影响因子：
64.8
作者：
Canman, JC;Cameron, LA;Salmon, ED
通讯作者：
Salmon, ED
影响因子：
9.2
作者：
Feierbach, B;Chang, F
通讯作者：
Chang, F