Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074.
Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074.
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DOI：
10.1111/1751-7915.12116
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发表时间：
2014-05
影响因子：
5.7
通讯作者：
Salas JA
中科院分区：
文献类型：
--
作者：
Olano C;García I;González A;Rodriguez M;Rozas D;Rubio J;Sánchez-Hidalgo M;Braña AF;Méndez C;Salas JA
白色链霉菌J1074是一株广泛用作次级代谢产物基因簇表达宿主的链霉菌。 对该生物体基因组的生物信息学分析预测存在27个次级代谢物基因簇。我们使用了三种不同的策略来激活S. albus J1074中的一些沉默/隐藏基因簇 ：两种杂合聚酮-非核糖体肽（PK-NRP）（抗霉素和6-epi-alteramides）、I型PK（杀念菌素）、非核糖体肽（NRP）（靛蓝）和糖基化化合物（保霉素）。通过在两个簇的选定基因前插入强的组成型启动子，激活了蓝色色素靛蓝和多环四酸酯大环内酰胺家族的两个新成员（6-epi-alteramides A和B）的产生。来自同一生物体的正调控基因的过表达也激活了6-epi-alteramides的生物合成，并且匹马菌素簇的调控基因pimM的异源表达激活了杀念菌素和抗霉素的同时生产，这表明两个簇之间存在某种交叉调节。糖基化化合物（保霉素）的集群也确定了通过比较的野生型菌株的高性能液相色谱曲线与突变体，其中两个关键酶的集群同时删除。
Streptomyces albus J1074 is a streptomycete strain widely used as a host for expression of secondary metabolite gene clusters. Bioinformatic analysis of the genome of this organism predicts the presence of 27 gene clusters for secondary metabolites. We have used three different strategies for the activation of some of these silent/cryptic gene clusters in S. albus J1074: two hybrid polyketide-non-ribosomal peptides (PK-NRP) (antimycin and 6-epi-alteramides), a type I PK (candicidin), a non-ribosomal peptides (NRP) (indigoidine) and glycosylated compounds (paulomycins). By insertion of a strong and constitutive promoter in front of selected genes of two clusters, production of the blue pigment indigoidine and of two novel members of the polycyclic tetramate macrolactam family (6-epi-alteramides A and B) was activated. Overexpression of positive regulatory genes from the same organism also activated the biosynthesis of 6-epi-alteramides and heterologous expression of the regulatory gene pimM of the pimaricin cluster activated the simultaneous production of candicidins and antimycins, suggesting some kind of cross-regulation between both clusters. A cluster for glycosylated compounds (paulomycins) was also identified by comparison of the high-performance liquid chromatography profiles of the wild-type strain with that of a mutant in which two key enzymes of the cluster were simultaneously deleted.
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影响因子：
14.9
作者：
Blin K;Medema MH;Kazempour D;Fischbach MA;Breitling R;Takano E;Weber T
通讯作者：
Weber T
影响因子：
2.8
作者：
Challis, Gregory L.
通讯作者：
Challis, Gregory L.
DOI：
10.1099/00221287-144-6-1465
发表时间：
1998-06-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Chater, KF
通讯作者：
Chater, KF
影响因子：
2.8
作者：
Campelo, AB;Gil, JA
通讯作者：
Gil, JA
影响因子：
3.3
作者：
ARGOUDELIS, AD;BACZYNSKYJ, L;SHILLIDAY, FB
通讯作者：
SHILLIDAY, FB