An easy-to-build and re-usable microfluidic system for live-cell imaging.
An easy-to-build and re-usable microfluidic system for live-cell imaging.
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DOI：
10.1186/s12860-018-0158-z
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发表时间：
2018-06-20
期刊：
影响因子：
--
通讯作者：
Coudreuse D
中科院分区：
文献类型：
--
作者：
Babic J;Griscom L;Cramer J;Coudreuse D
实时监测细胞对环境动态变化或特定治疗的反应已成为细胞生物学的核心。然而，当与活细胞成像相结合时，这种策略难以以精确和高时间分辨率实现，并且同时改变多个参数是一个重大挑战。最近，微流体技术为此类分析提供了强大的解决方案，对显微镜下观察的细胞生长的条件和培养基进行了前所未有的控制。然而，这种技术仍然没有得到充分利用，这主要是由于与微制造过程相关的复杂性。在这项研究中，我们开发了简单但功能强大的微流体设备，致力于活细胞成像。这些微系统利用了一种坚固的弹性体，研究人员可以随时获得这种弹性体，并且这种弹性体具有优异的粘合性能，特别是与显微镜级盖玻片的粘合性能。重要的是，这些芯片易于构建，无需复杂的设备，并且它们与复杂的定制流体网络的集成以及在单个设备上多路复用独立测定兼容。我们证明了芯片是可重复使用的，这是微流体技术在细胞生物学中推广的一个显著优势。此外，我们证明，它们允许动态，准确和同时控制的多个参数的细胞环境。虽然它们不具备使用复杂和昂贵的程序构建的微器件的所有功能，但我们开发的芯片的简单性和多功能性使它们成为一系列应用的有吸引力的替代品。这种设备的出现，它可以被任何实验室制造和使用，将为更多的研究团队提供充分利用这些新方法来研究细胞生物学的可能性。本文的在线版本（10.1186/s12860-018-0158-z）包含补充材料，可供授权用户使用。
Real-time monitoring of cellular responses to dynamic changes in their environment or to specific treatments has become central to cell biology. However, when coupled to live-cell imaging, such strategies are difficult to implement with precision and high time resolution, and the simultaneous alteration of multiple parameters is a major challenge. Recently, microfluidics has provided powerful solutions for such analyses, bringing an unprecedented level of control over the conditions and the medium in which cells under microscopic observation are grown. However, such technologies have remained under-exploited, largely as a result of the complexity associated with microfabrication procedures. In this study, we have developed simple but powerful microfluidic devices dedicated to live-cell imaging. These microsystems take advantage of a robust elastomer that is readily available to researchers and that presents excellent bonding properties, in particular to microscopy-grade glass coverslips. Importantly, the chips are easy-to-build without sophisticated equipment, and they are compatible with the integration of complex, customized fluidic networks as well as with the multiplexing of independent assays on a single device. We show that the chips are re-usable, a significant advantage for the popularization of microfluidics in cell biology. Moreover, we demonstrate that they allow for the dynamic, accurate and simultaneous control of multiple parameters of the cellular environment. While they do not possess all the features of the microdevices that are built using complex and costly procedures, the simplicity and versatility of the chips that we have developed make them an attractive alternative for a range of applications. The emergence of such devices, which can be fabricated and used by any laboratory, will provide the possibility for a larger number of research teams to take full advantage of these new methods for investigating cell biology. The online version of this article (10.1186/s12860-018-0158-z) contains supplementary material, which is available to authorized users.
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DOI：
10.1038/nrm4041
发表时间：
2015-09
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Duncombe TA;Tentori AM;Herr AE
通讯作者：
Herr AE
在药物发现的边界，片上的器官。
DOI：
10.1038/nrd4539
发表时间：
2015-04
期刊：
Nature reviews. Drug discovery
影响因子：
--
作者：
Esch EW;Bahinski A;Huh D
通讯作者：
Huh D
DOI：
10.1016/j.snb.2013.10.002
发表时间：
2014-03-01
期刊：
Sensors and actuators. B, Chemical
影响因子：
--
作者：
Bell L;Seshia A;Lando D;Laue E;Palayret M;Lee SF;Klenerman D
通讯作者：
Klenerman D
影响因子：
6.1
作者：
Mirzaei, Maryam;Pla-Roca, Mateu;Juncker, David
通讯作者：
Juncker, David
影响因子：
46.9
作者：
Bhatia, Sangeeta N.;Ingber, Donald E.
通讯作者：
Ingber, Donald E.