Wolbachia in the spittlebug Prosapia ignipectus: Variable infection frequencies, but no apparent effect on host reproductive isolation.
Wolbachia in the spittlebug Prosapia ignipectus: Variable infection frequencies, but no apparent effect on host reproductive isolation.
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DOI：
10.1002/ece3.7782
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发表时间：
2021-08
影响因子：
2.6
通讯作者：
Cooper BS
中科院分区：
文献类型：
--
作者：
Wheeler TB;Thompson V;Conner WR;Cooper BS
动物是复杂微生物群落的宿主，包括生活在它们细胞内的内共生体。沃尔巴克氏体细菌可能是最常见的内共生体，操纵宿主繁殖繁殖。许多沃尔巴克氏体引起细胞质不相容性（CI），当未感染的雌性与感染的雄性交配时，会导致卵孵化减少。导致强烈CI的沃尔巴克氏体传播到高和相对稳定的频率，而导致弱CI或无CI的菌株往往以中间，经常可变的频率持续存在。沃尔巴克氏体也可能有助于宿主生殖隔离（RI），尽管目前对这种贡献的支持仅限于少数系统。为了测试沃尔巴克氏体的频率变化和对主机RI的影响，我们采样了几个当地的Prosapia ignipectus（惠誉）（半翅目：Cercopidae）喷沫虫种群在美国东北部超过两年，包括密切并列的缅因州人口与不同的单态颜色形式，“黑色”和“内衬”。我们发现了一种感染P. ignipectus的B组沃尔巴克氏体（wPig），它与果蝇中的A组沃尔巴克氏体样模型wMel和wRi菌株不同-6至46 MYA。来自夏威夷和佛罗里达的姐妹种Prosapia bicincta（Say）的种群未被感染，这表明P. ignipectus在最初的分歧后获得了wPig。wPig的频率普遍较高，不同地点和年份之间存在差异。虽然表型分析wPig对宿主繁殖的影响目前尚不可行，但wPig基因组含有三组不同的CI基因座，与高wPig频率一致。最后，缅因州的单态黑色和单态内衬种群的P. ignipectus共享wPig和mtDNA单倍型，这意味着没有明显的影响wPig的维持这种形态接触区。我们假设P. ignipectus收购wPig水平观察到许多果蝇物种，和显着的CI和可变的传输产生高，但可变的wPig频率。我们报告说，一组B沃尔巴克氏体（wPig）感染喷沫虫Prosapia ignipectus。wPig可能是最近获得的，对美国缅因州的P. ignipectus形态接触区的维持几乎没有明显影响。wPig基因组包含三组与细胞质不相容性（CI）相关的基因座，我们假设，显着的CI和可变的传输在自然界中产生高但可变的wPig频率。
Animals serve as hosts for complex communities of microorganisms, including endosymbionts that live inside their cells. Wolbachia bacteria are perhaps the most common endosymbionts, manipulating host reproduction to propagate. Many Wolbachia cause cytoplasmic incompatibility (CI), which results in reduced egg hatch when uninfected females mate with infected males. Wolbachia that cause intense CI spread to high and relatively stable frequencies, while strains that cause weak or no CI tend to persist at intermediate, often variable, frequencies. Wolbachia could also contribute to host reproductive isolation (RI), although current support for such contributions is limited to a few systems. To test for Wolbachia frequency variation and effects on host RI, we sampled several local Prosapia ignipectus (Fitch) (Hemiptera: Cercopidae) spittlebug populations in the northeastern United States over two years, including closely juxtaposed Maine populations with different monomorphic color forms, “black” and “lined.” We discovered a group‐B Wolbachia (wPig) infecting P. ignipectus that diverged from group‐A Wolbachia—like model wMel and wRi strains in Drosophila—6 to 46 MYA. Populations of the sister species Prosapia bicincta (Say) from Hawaii and Florida are uninfected, suggesting that P. ignipectus acquired wPig after their initial divergence. wPig frequencies were generally high and variable among sites and between years. While phenotyping wPig effects on host reproduction is not currently feasible, the wPig genome contains three divergent sets of CI loci, consistent with high wPig frequencies. Finally, Maine monomorphic black and monomorphic lined populations of P. ignipectus share both wPig and mtDNA haplotypes, implying no apparent effect of wPig on the maintenance of this morphological contact zone. We hypothesize P. ignipectus acquired wPig horizontally as observed for many Drosophila species, and that significant CI and variable transmission produce high but variable wPig frequencies. We report that a group‐B Wolbachia (wPig) infects the spittlebug Prosapia ignipectus. wPig was likely recently acquired and has little apparent effect on the maintenance of a P. ignipectus morphological contact zone in Maine, USA. The wPig genome contains three sets of loci associated with cytoplasmic incompatibility (CI), and we hypothesize that significant CI and variable transmission produce high but variable wPig frequencies in nature.
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影响因子：
3.3
作者：
Cooper, Brandon S.;Vanderpool, Dan;Turelli, Michael
通讯作者：
Turelli, Michael
影响因子：
2.6
作者：
Conner WR;Blaxter ML;Anfora G;Ometto L;Rota-Stabelli O;Turelli M
通讯作者：
Turelli M
影响因子：
6.7
作者：
Ahmed MZ;Li SJ;Xue X;Yin XJ;Ren SX;Jiggins FM;Greeff JM;Qiu BL
通讯作者：
Qiu BL
影响因子：
3.7
作者：
Foottit RG;Maw E;Hebert PD
通讯作者：
Hebert PD
影响因子：
4.9
作者：
Hamm CA;Begun DJ;Vo A;Smith CC;Saelao P;Shaver AO;Jaenike J;Turelli M
通讯作者：
Turelli M