DNA barcodes for Nearctic Auchenorrhyncha (Insecta: Hemiptera).

DNA barcodes for Nearctic Auchenorrhyncha (Insecta: Hemiptera).
复制标题

DOI:
10.1371/journal.pone.0101385
复制
发表时间:
2014
期刊:
影响因子:
3.7
通讯作者:
Hebert PD
Hebert PD
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Foottit RG;Maw E;Hebert PD

文献摘要

参考文献

被引文献

相似文献

许多研究表明,线粒体细胞色素C氧化酶I(COI)基因5‘端的序列变异适合作为DNA条形码在广泛的动物种群中进行物种鉴定。为了评估DNA条形码在这一群体中的有效性,我们研究了来自加拿大国家昆虫保藏中心的标本中的半翅目:凹翅亚目147属471种。对COI基因的分析表明,在所研究的分类群中,93%的种内差异不到2%,而在70%的同属种对中,最小种间距离超过2%。虽然大多数物种的特征是不同的序列簇,但许多密切相关物种的成员的序列要么共享序列,要么显示出密切的相似性,25%的物种与其最近的邻居物种的距离不到1%。这项研究虽然是初步的,但提供了约8%的这种半翅目亚目物种的DNA条形码,这些物种发现于墨西哥以北的北美。条形码可以识别许多种,但不能区分某些物种组的成员。未来DNA条形码在监管、虫害管理和环境应用中的应用将成为可能,因为Auchenorribrncha的条形码库将扩展到包括更多物种和更广泛的地理覆盖范围。
Many studies have shown the suitability of sequence variation in the 5′ region of the mitochondrial cytochrome c oxidase I (COI) gene as a DNA barcode for the identification of species in a wide range of animal groups. We examined 471 species in 147 genera of Hemiptera: Auchenorrhyncha drawn from specimens in the Canadian National Collection of Insects to assess the effectiveness of DNA barcoding in this group. Analysis of the COI gene revealed less than 2% intra-specific divergence in 93% of the taxa examined, while minimum interspecific distances exceeded 2% in 70% of congeneric species pairs. Although most species are characterized by a distinct sequence cluster, sequences for members of many groups of closely related species either shared sequences or showed close similarity, with 25% of species separated from their nearest neighbor by less than 1%. This study, although preliminary, provides DNA barcodes for about 8% of the species of this hemipteran suborder found in North America north of Mexico. Barcodes can enable the identification of many species of Auchenorrhyncha, but members of some species groups cannot be discriminated. Future use of DNA barcodes in regulatory, pest management, and environmental applications will be possible as the barcode library for Auchenorrhyncha expands to include more species and broader geographic coverage.
DOI: 10.1098/rsbl.2003.0025
发表时间: 2003-08-07
影响因子: 4.7
作者:
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者: deWaard, JR
DOI: 10.1111/j.1755-0998.2010.02945.x
发表时间: 2011-03-01
影响因子: 7.7
作者:
Jung, Sunghoon;Duwal, Ram K.;Lee, Seunghwan
通讯作者: Lee, Seunghwan
DOI: 10.3958/059.035.0303
发表时间: 2010-09-01
影响因子: 0.4
作者:
Hudson, Andrinae;Richman, David B.;Creamer, Rebecca
通讯作者: Creamer, Rebecca
DOI: 10.1111/j.1755-0998.2009.02644.x
发表时间: 2009-05-01
影响因子: 7.7
作者:
Foottit, R. G.;Maw, H. E. L.;Montgomery, M. E.
通讯作者: Montgomery, M. E.
DOI: 10.1111/j.1365-3113.2009.00509.x
发表时间: 2010-04-01
影响因子: 4.8
作者:
Lee, Young June;Hill, Kathy B. R.
通讯作者: Hill, Kathy B. R.