Whole-Genome Sequencing of a Potential Ester-Synthesizing Bacterium Isolated from Fermented Golden Pomfret and Identification of Its Lipase Encoding Genes.
Whole-Genome Sequencing of a Potential Ester-Synthesizing Bacterium Isolated from Fermented Golden Pomfret and Identification of Its Lipase Encoding Genes.
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一株产酯细菌的全基因组测序及其脂肪酶基因的鉴定
DOI：
10.3390/foods11131954
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发表时间：
2022-06-30
期刊：
影响因子：
5.2
通讯作者：
Wang, Yueqi
中科院分区：
文献类型：
--
作者：
Wang, Huifang;Wu, Yanyan;Wang, Yueqi
微生物酯酶在食品工业中被认为是有价值的催化剂。从传统发酵金鲳鱼中分离到一株产酯酶的威尼斯不动杆菌SCSMX-3。它在中温、低盐和弱碱性环境中表现出良好的生长。由SCSMX-3产生的酯合酶在pH 8.0和35 °C下显示最大活性。基因组测序结果表明，该菌株含有一条336313 bp的环状染色体和两个环状质粒（质粒A-14424 bp和质粒B-11249 bp）。六种CRISPR结构增强了SCSMX-3的基因组稳定性，并为创造新的功能菌株提供了机会。基因功能分析表明，SCSMX-3产生在不同条件下生存和风味物质合成所必需的酶。此外，通过NCBI非冗余蛋白质数据库鉴定了49个编码与脂质代谢相关的酶的基因，包括三种三酰甘油脂肪酶和两种酯酶。由基因0302编码的脂肪酶属于GX组和abH 15超家族的abH15.02（洋葱伯克霍尔德菌脂肪酶）同系物。本研究结果为进一步了解中国对虾的基因组多样性和脂质代谢提供了理论依据.本研究为开发新的酯酶以改善发酵鱼制品风味奠定了基础。
Microbial ester synthases are regarded as valuable catalysts in the food industry. Here, one strain of Acinetobacter venetianus with ester synthase-production capacity, SCSMX-3, was isolated from traditional fermented golden pomfret. It exhibited good growth in mesophilic, low salt, and slightly alkaline environments. The ester synthase produced by SCSMX-3 displayed maximum activity at pH 8.0 and 35 °C. Genome sequencing revealed that the strain contains one circular chromosome of 336313 bp and two circular plasmids (plasmid A-14424 bp and plasmid B-11249 bp). Six CRISPR structures enhance the genomic stability of SCSMX-3 and provide the opportunity to create new functional strains. Gene function analysis indicated that SCSMX-3 produces the necessary enzymes for survival under different conditions and for flavor substance synthesis. Furthermore, 49 genes encoding enzymes associated with lipid metabolism, including three triacylglycerol lipases and two esterases, were identified through the NCBI Non-Redundant Protein Database. The lipase encoded by gene0302 belongs to the GX group and the abH15.02 (Burkholderia cepacia lipase) homolog of the abH15 superfamily. Our results shed light on the genomic diversity of and lipid metabolism in A. venetianus isolated from fermented golden pomfret, laying a foundation for the exploration of new ester synthases to improve the flavor of fermented fish products.
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影响因子：
14.9
作者：
Krawczyk PS;Lipinski L;Dziembowski A
通讯作者：
Dziembowski A
影响因子：
14.9
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
8.1
作者：
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通讯作者：
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影响因子：
3.7
作者：
Huang, Xiaodong;Gu, Yin;Gai, Chunlei
通讯作者：
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