Spatial distribution of D1R- and D2R-expressing medium-sized spiny neurons differs along the rostro-caudal axis of the mouse dorsal striatum.
Spatial distribution of D1R- and D2R-expressing medium-sized spiny neurons differs along the rostro-caudal axis of the mouse dorsal striatum.
复制标题
DOI：
10.3389/fncir.2013.00124
复制
发表时间：
2013
影响因子：
3.5
通讯作者：
Krieger P
中科院分区：
文献类型：
--
作者：
Gangarossa G;Espallergues J;Mailly P;De Bundel D;de Kerchove d'Exaerde A;Hervé D;Girault JA;Valjent E;Krieger P
纹状体投射神经元是纹状体黑质和纹状体苍白质中等大小的多棘神经元 (MSN)，分别优先表达 D1 (D1R) 和 D2 (D2R) 多巴胺受体。人们普遍认为这些神经元在物理上是混合的，没有细胞结构组织，尽管这尚未经过测试。为了解决这个问题，我们使用在 Drd1a 或 Drd2 启动子和空间点模式统计控制下表达增强型绿色荧光 (EGFP) 的 BAC 转基因小鼠。我们证明表达 D1R 和 D2R 的 MSN 随机分布在大部分背侧纹状体中，而尾侧纹状体中与 GPe 相邻的特定区域缺乏表达间接通路神经元标记的神经元。该区域几乎完全由表达 D1R 的 MSN 组成。这些神经元接收来自初级听觉皮层和内侧膝状丘脑核的兴奋性输入以及丰富的多巴胺神经支配。该区域包含胆碱能和 GABA 能中间神经元，但显然没有 D2R/A2aR 调节，因为在与报告小鼠杂交的 Drd2-EGFP 或 Drd2-Cre 以及 Adora-Cre BAC 转基因小鼠的神经毡中未检测到荧光。该纹状体区域表达钙结合蛋白 D28k、VGluT1 和 2，μ 阿片受体和前脑啡肽原含量较低。总而言之，在 D1R-MSN、D2R-MSN 和中间神经元密度中观察到的差异，以及表达 D1R 和 D2R/A2aR 的 MSN 的解剖分离表明纹状体的组织存在区域差异。
The striatum projection neurons are striatonigral and striatopallidal medium-sized spiny neurons (MSNs) that preferentially express D1 (D1R) and D2 (D2R) dopamine receptors, respectively. It is generally assumed that these neurons are physically intermingled, without cytoarchitectural organization although this has not been tested. To address this question we used BAC transgenic mice expressing enhanced green fluorescence (EGFP) under the control of Drd1a or Drd2 promoter and spatial point pattern statistics. We demonstrate that D1R- and D2R-expressing MSNs are randomly distributed in most of the dorsal striatum, whereas a specific region in the caudal striatum, adjacent to the GPe, lacks neurons expressing markers for indirect pathway neurons. This area comprises almost exclusively D1R-expressing MSNs. These neurons receive excitatory inputs from the primary auditory cortex and the medial geniculate thalamic nucleus and a rich dopamine innervation. This area contains cholinergic and GABAergic interneurons but apparently no D2R/A2aR modulation because no fluorescence was detected in the neuropil of Drd2-EGFP or Drd2-Cre, and Adora-Cre BAC transgenic mice crossed with reporter mice. This striatal area that expresses calbindin D28k, VGluT1 and 2, is poor in μ opiate receptors and preproenkephalin. Altogether, the differences observed in D1R-MSNs, D2R-MSNs, and interneurons densities, as well as the anatomical segregation of D1R- and D2R/A2aR-expressing MSNs suggest that there are regional differences in the organization of the striatum.
登录
查看更多内容
影响因子：
2.9
作者：
Crittenden JR;Graybiel AM
通讯作者：
Graybiel AM
影响因子：
4.1
作者：
Graybiel, AM
通讯作者：
Graybiel, AM
伏隔核中GABA能中型棘神经元的分布和分室组织。
影响因子：
3.5
作者：
Gangarossa G;Espallergues J;de Kerchove d'Exaerde A;El Mestikawy S;Gerfen CR;Hervé D;Girault JA;Valjent E
通讯作者：
Valjent E
影响因子：
56.9
作者：
GERFEN, CR;ENGBER, TM;SIBLEY, DR
通讯作者：
SIBLEY, DR
影响因子：
5.3
作者：
Ena, Sabrina L.;De Backer, Jean-Francois;d'Exaerde, Alban de Kerchove
通讯作者：
d'Exaerde, Alban de Kerchove