Infection state of Pantoea agglomerans in the rice water weevil Lissorhoptrus oryzophilus (Coleoptera: Curculionidae)
Infection state of Pantoea agglomerans in the rice water weevil Lissorhoptrus oryzophilus (Coleoptera: Curculionidae)
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稻水象甲 Lissorhoptrus oryzophilus（鞘翅目：象甲科）成团泛菌的感染状况
DOI：
10.1007/s13355-016-0433-4
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发表时间：
2016-08
影响因子：
1.3
通讯作者：
Jiang Mingxing
中科院分区：
文献类型：
--
作者：
Zhang Jingyu;Huang Yunshan;Huang Xu;Jiang Mingxing
聚集性泛菌是昆虫中一种重要的细菌共生体。尽管它经常在各种昆虫中被发现，但人们对它的菌株多样性和数量动态知之甚少。在本研究中，我们对北美和东亚地区的水稻重要害虫稻纵卷叶甲(鞘翅目：卷叶甲科)的细菌进行了研究。基于细菌16S rRNA基因的克隆和测序，我们从不同的象虫种群中鉴定了三个新的聚集性巴氏杆菌菌株，每个种群只有一个菌株。在雌性成虫中，肠道是聚集性巴氏杆菌的主要组织。这种细菌也存在于卵巢中，但密度很低。在所有未成熟阶段(从卵到蛹)，细菌的密度都保持在低水平；然而，随着成虫的出现，密度大幅增加。其密度在越冬期较低，但在越冬成虫被允许取食寄主植物后，即从早期发育到活跃繁殖阶段后，密度逐渐增加。在生殖后期，成虫的感染密度恢复到较低水平。综上所述，我们调查的每个稻瘟菌种群可能都被一种聚集性巴氏杆菌菌株感染，成虫的肠道是携带这种细菌的主要组织/发育阶段，活跃繁殖的成虫感染水平较高。
Pantoea agglomerans is an important bacterial symbiont of insects. Despite its frequent discovery in various insects, little is known of its strain diversity and quantitative dynamics. In this study, we investigated these aspects of the bacterium in the rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), a serious pest of rice in North America and East Asia. Based on the cloning and sequencing of a bacterial 16S rRNA gene, we identified three novel P. agglomerans strains from different weevil populations, with each population harboring only one strain. In female adults, gut was the dominant tissue harboring P. agglomerans. This bacterium was also present in the ovary, but its density was very low there. The bacterium was maintained at a low density across all immature stages (from egg to pupa); however, the density increased substantially as adults emerged. Its density was low in the overwintering stage, but increased stepwise after the overwintered adults were allowed to feed on host plants, i.e., when they developed from early to actively reproducing stages. The infection density returned to a low level in the adults at a late reproductive stage. In conclusion, each of the L. oryzophilus populations we investigated was probably infected by a single P. agglomerans strain, and the gut of adults is the major tissue/developmental stage to harbor this bacterium, with actively reproducing adults attaining a high level of infection.
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影响因子：
3.8
作者：
Heerman M;Weng JL;Hurwitz I;Durvasula R;Ramalho-Ortigao M
通讯作者：
Ramalho-Ortigao M
DOI：
--
发表时间：
1959
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
B. Greenberg
通讯作者：
B. Greenberg
影响因子：
4.4
作者：
T. Fukatsu;N. Nikoh
通讯作者：
T. Fukatsu;N. Nikoh
影响因子：
4
作者：
Crippen, T. L.;Zheng, L.;Yu, Z.
通讯作者：
Yu, Z.
影响因子：
2.6
作者：
R. Rădvan
通讯作者：
R. Rădvan