Necrosis and ethylene-inducing-like peptide patterns from crop pathogens induce differential responses within seven brassicaceous species.
Necrosis and ethylene-inducing-like peptide patterns from crop pathogens induce differential responses within seven brassicaceous species.
复制标题
作物病原体的坏死和乙烯诱导样肽模式在七种芸苔科植物中引起不同的反应。
DOI：
10.1111/ppa.13615
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发表时间：
2022-12
期刊：
影响因子：
2.7
通讯作者：
Ridout, Christopher J.
中科院分区：
文献类型：
--
作者：
Schoonbeek, Henk-jan;Yalcin, Hicret Asli;Burns, Rachel;Taylor, Rachel Emma;Casey, Adam;Holt, Sam;Van den Ackerveken, Guido;Wells, Rachel;Ridout, Christopher J.
关键词：
转化研究是推进植物免疫基础知识在作物改良中的应用所必需的。微生物/病原体相关分子模式（MAMPs/PAMPs）的识别触发了植物的第一层免疫。广泛存在的坏死和乙烯诱导肽1（NEP 1）样蛋白（NLP）家族包含被许多植物物种识别的免疫原性肽模式。拟南芥可以通过模式识别受体AtRLP23及其辅助受体SOBIR1、BAK1和BKK 1识别NLP，导致诱导防御反应，包括产生活性氧（ROS）和升高细胞内[Ca2 +]。然而，对芸苔属作物物种的NLP感知知之甚少。在12个不同的加入为每六个芸苔属作物物种，我们表现出响应灰葡萄孢NLP BcNEP2的变化，与甘蓝（CC基因组）是无响应的，只有两个甘蓝型油菜品种响应BcNEP2。来自这些作物物种的四种真菌病原体的肽在B中引起类似于BcNEP 2的反应。napus和Arabidopsis。在Atrlp23、Atsobir1和Atbak1 - 5 Atbkk1 - 1突变体中，NLP肽对ROS的诱导强烈降低，证实了对芸苔属病原体NLP的识别以与拟南芥中来自透明操纵孢菌的HaNLP 3相似的方式发生。两个B的基因组的计算机分析。甘蓝型油菜种质中AtBAK1、AtBKK1和AtSOBIR1的同源物相似，但AtRLP23同源物的组织存在差异。我们不能检测到对NLP肽的应答能力和对B的抗性之间的强相关性。灰色区域。除B外的所有芸苔属物种。与拟南芥不同，甘蓝识别病原体NLP与疾病控制无关，突出了从模型到作物的转化研究的挑战。
Translational research is required to advance fundamental knowledge on plant immunity towards application in crop improvement. Recognition of microbe/pathogen‐associated molecular patterns (MAMPs/PAMPs) triggers a first layer of immunity in plants. The broadly occurring family of necrosis‐ and ethylene‐inducing peptide 1 (NEP1)‐like proteins (NLPs) contains immunogenic peptide patterns that are recognized by a number of plant species. Arabidopsis can recognize NLPs by the pattern recognition receptor AtRLP23 and its co‐receptors SOBIR1, BAK1, and BKK1, leading to induction of defence responses including the production of reactive oxygen species (ROS) and elevation of intracellular [Ca2+]. However, little is known about NLP perception in Brassica crop species. Within 12 diverse accessions for each of six Brassica crop species, we demonstrate variation in response to Botrytis cinerea NLP BcNEP2, with Brassica oleracea (CC genome) being nonresponsive and only two Brassica napus cultivars responding to BcNEP2. Peptides derived from four fungal pathogens of these crop species elicited responses similar to BcNEP2 in B. napus and Arabidopsis. Induction of ROS by NLP peptides was strongly reduced in Atrlp23, Atsobir1 and Atbak1‐5 Atbkk1‐1 mutants, confirming that recognition of Brassica pathogen NLPs occurs in a similar manner to that of HaNLP3 from Hyaloperonospora arabidopsidis in Arabidopsis. In silico analysis of the genomes of two B. napus accessions showed similar presence of homologues for AtBAK1, AtBKK1 and AtSOBIR1 but variation in the organization of AtRLP23 homologues. We could not detect a strong correlation between the ability to respond to NLP peptides and resistance to B. cinerea. All Brassica species except B. oleracea recognize pathogen NLPs which, unlike Arabidopsis, was not correlated with disease control highlighting challenges of translational research from models to crops.
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影响因子：
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作者：
Bjornson M;Pimprikar P;Nürnberger T;Zipfel C
通讯作者：
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影响因子：
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Zipfel, Cyril
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Schoonbeek, Henk-jan;Wang, Hsi-Hua;Ridout, Christopher J.
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Ridout, Christopher J.
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NEI, M