Overexpression of BpERF1.1 in Betula Platyphylla enhanced tolerance to multiple abiotic stresses
Overexpression of BpERF1.1 in Betula Platyphylla enhanced tolerance to multiple abiotic stresses
复制标题
白桦中BpERF1.1的过度表达增强了对多种非生物胁迫的耐受性
DOI：
10.1007/s12298-022-01206-3
复制
发表时间：
2022-06
影响因子：
3.5
通讯作者：
Su Chen
中科院分区：
文献类型：
--
作者：
Xiang Zhang;Xin Lin;Song Chen;Su Chen
冷，盐和干旱是影响植物生长和作物产量的主要环境压力。 ERF1是经过良好研究的ERF基因家族的成员，已被证明在植物的非生物压力中起着重要作用。在这项研究中，在桦木中过表达ERF1，bperf1.1（betula plathylla suk。），并获得了11种转基因线。在BPERF1.1过表达的线和相应的WT线上检查了冷，盐和干旱处理的影响。与WT线相比，转基因线对这些非生物应力的耐受性提高。与WT相比，进一步的RNA-seq分析确定了转基因桦木中的689个差异表达的基因（DEG），包括228个上调的基因和461个下调基因。基因本体富集分析的结果表明，在这些DEG中，有273个基因参与了各种植物生物学过程，其中83％参与细胞过程，代谢过程，生物学调节和对刺激的反应（11％）。所有这些结果表明，bperf1.1基因可以通过与植物对非生物胁迫的植物反应涉及的其他基因互连而对桦木对冷，盐和干旱胁迫的耐受性和抗性。
Cold, salt and drought are the main environmental stresses affecting plant growth and crop yield. ERF1, a member of the well-studied ERF gene family, has been proven to play an important role in abiotic stress in plants. In this study, an ERF1, BpERF1.1, was overexpressed in birch (Betula Platyphylla Suk.) and 11 transgenic lines were obtained. Effects of cold, salt and drought treatments were checked on the BpERF1.1 overexpressing lines and the corresponding WT line. The transgenic lines showed improved tolerance against these abiotic stresses than the WT line. Further RNA-seq analysis identified 689 differentially expressed genes (DEGs) in the transgenic birch compared with WT, including 228 up-regulated genes and 461 down-regulated genes. The result of gene ontology enrichment analysis showed that among these DEGs, 273 genes were involved in various plant biological process, and 83% of them were involved in cellular process, metabolic process, biological regulation and response to stimulus (11%). All these results demonstrate that BpERF1.1 gene improves the tolerance and resistance of birch against cold, salt and drought stress, probably by interconnecting with other genes involved in plant response to abiotic stresses.
登录
查看更多内容
DOI：
10.1016/b978-0-12-809633-8.20106-4
发表时间：
2020-02
期刊：
--
影响因子：
--
作者：
A. Prjibelski;Anton I. Korobeynikov;Alla L. Lapidus
通讯作者：
A. Prjibelski;Anton I. Korobeynikov;Alla L. Lapidus
垂枝桦 NAC 基因家族的全基因组分析
影响因子：
2.9
作者：
Chen, Song;Lin, Xin;Chen, Su
通讯作者：
Chen, Su
影响因子：
6.9
作者：
Colombatti, Francisco;Mencia, Regina;Welchen, Elina
通讯作者：
Welchen, Elina
影响因子：
64.5
作者：
Zhu, Jian-Kang
通讯作者：
Zhu, Jian-Kang
影响因子：
3.5
作者：
Dhar, Nikhilesh;Caruana, Julie;Raina, Ramesh
通讯作者：
Raina, Ramesh