A robotic BG1Luc reporter assay to detect estrogen receptor agonists.
A robotic BG1Luc reporter assay to detect estrogen receptor agonists.
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DOI：
10.1016/j.tiv.2014.03.013
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发表时间：
2014-08
影响因子：
3.2
通讯作者：
Bittner, George D.
中科院分区：
文献类型：
--
作者：
Stoner, Matthew A.;Yang, Chun Z.;Bittner, George D.
具有雌激素活性（EA）的内分泌干扰化学物质与各种不良健康影响有关。负责评估体外转录激活测定以检测 EA 雌激素受体 (ER) 激动剂的美国机构 (ICCVAM/NICEATM) 最近验证了手动格式的 BG1Luc 测定，但更喜欢机器人格式。我们开发了一种机器人 BG1Luc EA 测定法来检测 EA，该测定法与 ICCVAM 荟萃分析和 27 种 ICCVAM 测试物质的手动格式的 ICCVAM BG1Luc 结果 100% 一致，即没有假阴性或假阳性。该机器人检测还一致地评估了其他更有问题的 ICCVAM 测试物质，例如克罗米芬柠檬酸盐、L-甲状腺素和他莫昔芬。使用这种机器人 BG1Luc 检测的激动剂反应始终受到 ER 拮抗剂 ICI 182,780 的抑制，证实激动剂反应是由于与 ER 结合而不是非特异性激动剂反应。该机器人检测还检测了复杂物质混合物中的 EA，例如个人护理产品、塑料树脂或塑料消费品的提取物。与手动版本或其他 ICCVAM/OECD 验证的 EA 检测（手动 BG1Luc 和 CERI）相比，这种机器人 BG1Luc 检测至少具有同样高的准确性、更高的灵敏度和可重复性。
Endocrine disrupting chemicals with estrogenic activity (EA) have been associated with various adverse health effects. US agencies (ICCVAM/NICEATM) tasked to assess in vitro transcription activation assays to detect estrogenic receptor (ER) agonists for EA have recently validated a BG1Luc assay in manual format, but prefer robotic formats. We have developed a robotic BG1Luc EA assay to detect EA that demonstrated 100% concordance with ICCVAM meta-analyses and ICCVAM BG1Luc results in manual format for 27 ICCVAM test substances, i.e. no false negatives or false positives. This robotic assay also consistently assessed other, more problematic ICCVAM test substances such as clomiphene citrate, L-thyroxin, and tamoxifen. Agonist responses using this robotic BG1Luc assay were consistently inhibited by the ER antagonist ICI 182,780, confirming that agonist responses were due to binding to ERs rather than to a non-specific agonist response. This robotic assay also detected EA in complex mixtures of substances such as extracts of personal care products, plastic resins or plastic consumer products. This robotic BG1Luc assay had at least as high accuracy and greater sensitivity and repeatability when compared to its manual version or to the other ICCVAM/OECD validated assays for EA (manual BG1Luc and CERI).
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影响因子：
3.4
作者：
Adewale HB;Todd KL;Mickens JA;Patisaul HB
通讯作者：
Patisaul HB
DOI：
10.1590/s1413-81232012000200015
发表时间：
2012-02-01
期刊：
Ciência & Saúde Coletiva
影响因子：
--
作者：
Vandenberg, Laura N.;Chahoud, Ibrahim;Schoenfelder, Gilbert
通讯作者：
Schoenfelder, Gilbert
影响因子：
3.8
作者：
Sanseverino, John;Eldridge, Melanie L.;Sayler, Gary S.
通讯作者：
Sayler, Gary S.
影响因子：
10.4
作者：
Calafat AM;Kuklenyik Z;Reidy JA;Caudill SP;Ekong J;Needham LL
通讯作者：
Needham LL
影响因子：
8.8
作者：
Schultis, T;Metzger, JW
通讯作者：
Metzger, JW