The impact of neonatal bisphenol-A exposure on sexually dimorphic hypothalamic nuclei in the female rat.
The impact of neonatal bisphenol-A exposure on sexually dimorphic hypothalamic nuclei in the female rat.
复制标题
DOI：
10.1016/j.neuro.2010.07.008
复制
发表时间：
2011-01
期刊：
影响因子：
3.4
通讯作者：
Patisaul HB
中科院分区：
文献类型：
--
作者：
Adewale HB;Todd KL;Mickens JA;Patisaul HB
由于其内分泌干扰特性，塑料成分双酚a（BPA）对人类健康的潜在威胁目前仍不清楚。BPA存在于大量聚碳酸酯塑料、食品和饮料容器以及医疗设备中，被认为与雌激素受体（ER）结合，从而干扰雌激素依赖性过程。我们的实验室先前已经表明，在新生儿关键期暴露于BPA（50 mg/kg bw或50μg/kg bw）与雌性Long-Evans大鼠的青春期提前、早期生殖衰老和卵巢畸形有关。在这里，使用从相同的动物获得的神经组织，我们探讨了新生儿BPA暴露对女性生殖生理学和行为的关键性二态性下丘脑区域的发展的影响。终点包括定量室旁核（PVN）中催产素免疫反应神经元（OT-ir）、腹内侧核腹外侧亚部（VMNvl）中5-HT-ir纤维密度以及内侧视前区（MPOA）、VMNvl和弓状核（ARC）中ERα-ir神经元数量。两种剂量的BPA都增加了PVN内OT-ir神经元的数量，但对任何分析区域的5-HT-ir纤维密度或ERα-ir神经元数量均无显著影响。除了下丘脑发育，我们还评估了女性的性行为和体重。没有观察到BPA对雌性动物的性感受性或性行为的影响。然而，在出生后第99天，用BPA处理的雌性动物的体重显著高于对照组雌性动物。BPA对体重的影响至关重要，因为新陈代谢的改变通常与生殖功能障碍有关。总的来说，本研究和我们先前的研究结果表明，新生儿BPA暴露在雌性大鼠下丘脑的影响是区域特异性的，并支持发育BPA暴露可能会对雌性生殖发育产生不利影响的假设。
Now under intense scrutiny, due to its endocrine disrupting properties, the potential threat the plastics component bisphenol-a (BPA) poses to human health remains unclear. Found in a multitude of polycarbonate plastics, food and beverage containers, and medical equipment, BPA is thought to bind to estrogen receptors (ERs), thereby interfering with estrogen-dependent processes. Our lab has previously shown that exposure to BPA (50mg/kg bw or 50μg/kg bw) during the neonatal critical period is associated with advancement of puberty, early reproductive senescence and ovarian malformations in female Long-Evans rats. Here, using neural tissue obtained from the same animals, we explored the impact of neonatal BPA exposure on the development of sexually dimorphic hypothalamic regions critical for female reproductive physiology and behavior. Endpoints included quantification of oxytocin-immunoreactive neurons (OT-ir) in the paraventricular nucleus (PVN), serotonin (5-HT-ir) fiber density in the ventrolateral subdivision of the ventromedial nucleus (VMNvl) as well as ERα-ir neuron number in the medial preoptic area (MPOA), the VMNvl, and the arcuate nucleus (ARC). Both doses of BPA increased the number of OT-ir neurons within the PVN, but no significant effects were seen on 5-HT-ir fiber density or ERα-ir neuron number in any of the areas analyzed. In addition to hypothalamic development, we also assessed female sex behavior and body weight. No effect of BPA on sexual receptivity or proceptive behavior in females was observed. Females treated with BPA, however, weighed significantly more than control females by postnatal day 99. This effect of BPA on weight is critical because alterations in metabolism, are frequently associated with reproductive dysfunction. Collectively, the results of this and our prior study indicate that the impact of neonatal BPA exposure within the female rat hypothalamus is region specific and support the hypothesis that developmental BPA exposure may adversely affect reproductive development in females.
登录
查看更多内容
新生儿重症监护室早产儿接触双酚A和其他酚类物质。
影响因子：
10.4
作者：
Calafat, Antonia M.;Weuve, Jennifer;Ye, Xiaoyun;Jia, Lily T.;Hu, Howard;Ringer, Steven;Huttner, Ken;Hauser, Russ
通讯作者：
Hauser, Russ
DOI：
10.1073/pnas.95.6.3281
发表时间：
1998-03-17
影响因子：
11.1
作者：
Alves, SE;Lopez, V;Weiland, NG
通讯作者：
Weiland, NG
影响因子：
3.8
作者：
Benachour, Nora;Aris, Aziz
通讯作者：
Aris, Aziz
影响因子：
10.4
作者：
Calafat AM;Kuklenyik Z;Reidy JA;Caudill SP;Ekong J;Needham LL
通讯作者：
Needham LL
影响因子：
3.2
作者：
BROAD, KD;KENDRICK, KM;KEVERNE, EB
通讯作者：
KEVERNE, EB