Generation of functional fat organoid from rat superficial fascia.
Generation of functional fat organoid from rat superficial fascia.
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从大鼠浅筋膜中生成功能性脂肪类器官
DOI：
10.1080/21623945.2022.2072446
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发表时间：
2022-12
期刊：
影响因子：
3.3
通讯作者：
中科院分区：
文献类型：
--
作者：
类器官是由细胞类型、组织结构和生物学功能与天然器官相似的自组织组织或细胞在体外形成的三维细胞结构。根据独特的器官结构和细胞类型，生产有机化合物需要个性化的设计，而且仍然具有挑战性。包括脂肪组织在内的某些组织的有机体仍有待生成，以便在结构和功能上更忠实于其原始器官。我们先前建立了脂肪细胞起源于非脂肪筋膜组织的新模型。在这里，我们研究了3D水凝胶中的浅筋膜片段，发现它们能够转化为相对较大的脂肪细胞聚集体，其中包含成熟的单眼脂肪细胞，这实际上是“脂肪器官”。这些筋膜来源的脂肪有机体具有典型的脂肪组织结构，具有脂肪细胞合成、储存、水解甘油三酯和分泌脂肪因子的主要功能。从浅筋膜中产生脂肪有机体可以为脂肪细胞的研究提供新的途径，并有力地证明脂肪组织和细胞都起源于筋膜。我们的发现深入了解了不同器官和组织之间的相互作用对代谢调节的影响，并为研究肥胖、糖尿病和其他代谢性疾病的新疗法提供了新的知识。缩写：3D：三维；ASC：脂肪来源的基质细胞；C/EBP：CCAAT增强子结合蛋白；EDU：5-乙炔-2-脱氧尿苷；FABP：脂肪酸结合蛋白4；Fas：脂肪酸合成酶；FSC：筋膜来源的基质细胞；Plin1：Perilipin-1；Plin2：Perilipin-2；PPARγ：过氧化物酶体增殖物激活的受体γ；WAT：白色脂肪组织
The organoid is a 3D cell architecture formed by self-organized tissues or cells in vitro with similar cell types, histological structures, and biological functions of the native organ. Depending on the unique organ structures and cell types, producing organoids requires individualized design and is still challenging. Organoids of some tissues, including adipose tissue, remain to generate to be more faithful to their original organ in structure and function. We previously established a new model of the origin of adipose cells originating from non-adipose fascia tissue. Here, we investigated superficial fascia fragments in 3D hydrogel and found they were able to transform into relatively large adipocyte aggregates containing mature unilocular adipocytes, which were virtually “fat organoids”. Such fascia-originated fat organoids had a typical structure of adipose tissues and possessed the principal function of adipose cells in the synthesis, storage, hydrolysis of triglycerides and adipokines secretion. Producing fat organoids from superficial fascia can provide a new approach for adipocyte research and strongly evidences that both adipose tissues and cells originate from fascia. Our findings give insights into metabolic regulation by the crosstalk between different organs and tissues and provide new knowledge for investigating novel treatments for obesity, diabetes and other metabolic diseases. Abbreviations: 3D: three dimensional; ASC: adipose-derived stromal cells; C/EBP: CCAAT-enhancer-binding protein; EdU: 5-ethynyl-2-deoxyuridine; FABP4: fatty acid-binding protein 4; FAS: fatty acid synthase; FSCs: fascia-derived stromal cells; Plin1: perilipin-1; Plin2: perilipin-2; PPARγ: peroxisome proliferator-activated receptor γ; WAT: white adipose tissue
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影响因子：
10
作者：
Abbott RD;Wang RY;Reagan MR;Chen Y;Borowsky FE;Zieba A;Marra KG;Rubin JP;Ghobrial IM;Kaplan DL
通讯作者：
Kaplan DL
DOI：
10.1002/stem.2852
发表时间：
2018-09
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Kaushik G;Ponnusamy MP;Batra SK
通讯作者：
Batra SK
影响因子：
5.6
作者：
Scioli MG;Bielli A;Gentile P;Mazzaglia D;Cervelli V;Orlandi A
通讯作者：
Orlandi A
影响因子：
5.5
作者：
Li, Guangheng;Zheng, Bo;Huard, Johnny
通讯作者：
Huard, Johnny
DOI：
10.1007/978-1-61737-960-4_23
发表时间：
2011-01-01
期刊：
ADIPOSE-DERIVED STEM CELLS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Choi, Jennifer H.;Bellas, Evangelia;Kaplan, David L.
通讯作者：
Kaplan, David L.