Starvation selection reduces and delays larval ecdysone production and signaling.
Starvation selection reduces and delays larval ecdysone production and signaling.
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DOI：
10.1242/jeb.246144
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发表时间：
2023-09-15
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影响因子：
--
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先前的研究表明，黑腹果蝇的饥饿抵抗能力会导致羽化延迟和成年脂肪储存增加。假设这些性状是由饥饿选择压力引起的，但其机制尚不清楚。我们发现，我们的饥饿选择（SS）种群在幼虫发育过程中储存了更多的脂肪，并延长了幼虫发育和蛹的发育时间。与蜕皮类固醇激素脉冲相关的第三龄发育检查点越来越延迟。 SS 种群中幼虫后期的延迟表明蜕皮激素信号减弱和延迟。蜕皮类固醇（与代谢活性 20-羟基蜕皮激素和 α-蜕皮激素前体具有最大亲和力）的酶免疫测定证实，与喂养对照（FC）果蝇相比，SS 群体的激素产生减少并延迟。用补充有α-蜕皮激素的食物喂养三龄幼虫可以部分挽救发育迟缓并降低随后成虫的饥饿抵抗力。这项工作表明，饥饿选择会导致幼虫阶段蜕皮类固醇的产生减少和延迟，并影响发育延迟表型，从而有助于随后的成年脂肪储存和饥饿抵抗。摘要：饥饿选择的进化可以减少和延迟协调发育过渡的激素，并延长果蝇的幼虫和蛹期。
Previous studies have shown that selection for starvation resistance in Drosophila melanogaster results in delayed eclosion and increased adult fat stores. It is assumed that these traits are caused by the starvation selection pressure, but its mechanism is unknown. We found that our starvation-selected (SS) population stores more fat during larval development and has extended larval development and pupal development time. Developmental checkpoints in the third instar associated with ecdysteroid hormone pulses are increasingly delayed. The delay in the late larval period seen in the SS population is indicative of reduced and delayed ecdysone signaling. An enzyme immunoassay for ecdysteroids (with greatest affinity to the metabolically active 20-hydroxyecdysone and the α-ecdysone precursor) confirmed that the SS population had reduced and delayed hormone production compared with that of fed control (FC) flies. Feeding third instar larvae on food supplemented with α-ecdysone partially rescued the developmental delay and reduced subsequent adult starvation resistance. This work suggests that starvation selection causes reduced and delayed production of ecdysteroids in the larval stage and affects the developmental delay phenotype that contributes to subsequent adult fat storage and starvation resistance. Summary: Evolution from starvation selection can reduce and delay hormones that coordinate developmental transitions and extend the larval and pupal period in the fruit fly.
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影响因子：
3.7
作者：
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通讯作者：
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