Comparative Transcriptome Analyses of Different Rheum officinale Tissues Reveal Differentially Expressed Genes Associated with Anthraquinone, Catechin, and Gallic Acid Biosynthesis.
Comparative Transcriptome Analyses of Different Rheum officinale Tissues Reveal Differentially Expressed Genes Associated with Anthraquinone, Catechin, and Gallic Acid Biosynthesis.
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不同大黄组织的比较转录组分析揭示了与蒽醌、儿茶素和没食子酸生物合成相关的差异表达基因
作者：
大黄。是一种重要的传统中草药，其干燥的根和根茎被广泛用于治疗多种疾病。然而，以往的研究主要集中在对其活性成分及其药理作用的研究上，对其生物合成的分子机制尚不清楚。在这里，我们进行了比较转录组分析，以阐明根、茎和叶中的差异表达基因（DEGs）。共生成N50为769 bp的236,031个unigenes，其中136,329个（57.76%）被注释。经不同组织对比分析，共鉴定出5884个deg；在蒽醌、儿茶素/没食子酸生物合成途径中分别发现175个和126个具有组织特异性表达的关键酶基因，并对部分关键酶基因进行了qRT-PCR验证。蓼科PKSIII家族的系统发育表明，可能只有PL_741 PKSIII1、PL_11549 PKSIII5和PL_101745 PKSIII6在聚酮途径中编码PKSIII。这些研究结果将揭示黄皮次生代谢物组织特异性积累和调控的分子基础，为今后黄皮遗传多样性、分子辅助育种和种质资源改良奠定基础。
Rheum officinale Baill. is an important traditional Chinese medicinal herb, its dried roots and rhizomes being widely utilized to cure diverse diseases. However, previous studies mainly focused on the active compounds and their pharmacological effects, and the molecular mechanism underlying the biosynthesis of these ingredients in R. officinale is still elusive. Here, we performed comparative transcriptome analyses to elucidate the differentially expressed genes (DEGs) in the root, stem, and leaf of R. officinale. A total of 236,031 unigenes with N50 of 769 bp was generated, 136,329 (57.76%) of which were annotated. A total of 5884 DEGs was identified after the comparative analyses of different tissues; 175 and 126 key enzyme genes with tissue-specific expression were found in the anthraquinone, catechin/gallic acid biosynthetic pathway, respectively, and some of these key enzyme genes were verified by qRT-PCR. The phylogeny of the PKS III family in Polygonaceae indicated that probably only PL_741 PKSIII1, PL_11549 PKSIII5, and PL_101745 PKSIII6 encoded PKSIII in the polyketide pathway. These results will shed light on the molecular basis of the tissue-specific accumulation and regulation of secondary metabolites in R. officinale, and lay a foundation for the future genetic diversity, molecular assisted breeding, and germplasm resource improvement of this essential medicinal plant.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
DOI：
10.1016/j.cbd.2015.11.003
发表时间：
2016-03-01
影响因子：
3
作者：
Das, Sunetra;Pitts, Natalie L.;Mykles, Donald L.
通讯作者：
Mykles, Donald L.
影响因子：
4.9
作者：
Cao YJ;Pu ZJ;Tang YP;Shen J;Chen YY;Kang A;Zhou GS;Duan JA
通讯作者：
Duan JA
影响因子：
5.6
作者：
Barco, Brenden;Clay, Nicole K.
通讯作者：
Clay, Nicole K.
塞纳塞纳西氏症的从头转录组组装阐明了蒽醌的生物合成途径。
影响因子：
5.6
作者：
Kang SH;Lee WH;Sim JS;Thaku N;Chang S;Hong JP;Oh TJ
通讯作者：
Oh TJ