Platelet-mediated adhesion of Staphylococcus epidermidis to hydrophobic NHLBI reference polyethylene.
Platelet-mediated adhesion of Staphylococcus epidermidis to hydrophobic NHLBI reference polyethylene.
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血小板介导的表皮葡萄球菌与疏水性 NHLBI 参考聚乙烯的粘附。
DOI：
10.1002/jbm.820270902
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Marchant,RE
中科院分区：
文献类型：
--
作者：
Wang,IW;Anderson,JM;Marchant,RE
利用旋转圆盘系统模拟人体血液循环的血流动力学，定量研究了血小板和血浆蛋白对表皮葡萄球菌RP62A与疏水性NHLBI参考聚乙烯黏附的影响。细菌的粘附通过粘附系数来量化，粘附系数是细菌转移到表面的百分比。结果表明，与裸露的聚合物表面相比，通过吸附血浆蛋白进行表面修饰可降低表皮s的粘附性。即使在最高的生理剪切应力水平下，这种表面修饰也不足以完全消除细菌粘附。S表皮与经血小板修饰的聚乙烯表面有很强的粘附性。这很明显，因为大约50%的贴壁表皮与仅占表面面积4%的接触活化血小板结合。在所研究的生理剪切条件范围内，血小板的粘附系数显著大于蛋白质吸附聚乙烯表面的粘附系数至少一个数量级（P≤0.01）。这些研究表明，是接触活化血小板的生物表面修饰，而不是血浆蛋白，介导了表皮对聚乙烯的粘附。©1993 John Wiley & Sons, Inc
The effects of platelets and plasma proteins on the adhesion ofStaphylococcus epidermidisstrain RP62A to hydrophobic NHLBI reference polyethylene was quantitatively studied using a rotating disk system to generate well‐defined shear conditions simulating the hemodynamics of human blood circulation. Bacterial adhesion was quantified by adhesive coefficient, the percentage of bacteria transported to the surface that becomes adherent. The results showed that surface modification by adsorption of plasma proteins reduced the adhesion ofS epidermidisas compared to the bare polymer surface. This surface modification was not sufficient to eliminate completely bacterial adhesion, even at the highest physiologic shear stress level.S epidermidisdid adhere strongly to polyethylene surface modified by platelets. This is readily evident as approximately 50% of the adherentS epidermidiswere bound to contact‐activated platelets which occupied only 4% of the surface area. Adhesive coefficients to platelets were significantly greater than to the protein‐adsorbed polyethylene surface by at least one order of magnitude (P≤ .01) across the range of physiological shear conditions investigated. These studies show that it is biologic surface modification by contact‐activated platelets, and not plasma proteins, which mediatesS epidermidisadhesion to polyethylene. © 1993 John Wiley & Sons, Inc.
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影响因子：
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作者：
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DOI：
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影响因子：
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作者：
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DOI：
10.1002/jbm.820250311
发表时间：
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期刊：
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影响因子：
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