Green Synthesis of Gold and Silver Nanoparticles Using Leaf Extract of Capsicum chinense Plant.
Green Synthesis of Gold and Silver Nanoparticles Using Leaf Extract of Capsicum chinense Plant.
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DOI：
10.3390/molecules27051692
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发表时间：
2022-03-04
期刊：
影响因子：
--
通讯作者：
Velázquez-Juárez G
中科院分区：
文献类型：
--
作者：
Lomelí-Rosales DA;Zamudio-Ojeda A;Reyes-Maldonado OK;López-Reyes ME;Basulto-Padilla GC;Lopez-Naranjo EJ;Zuñiga-Mayo VM;Velázquez-Juárez G
到目前为止，几项研究集中在利用辣椒属植物果实的提取物合成金属纳米颗粒。然而，由于果实是植物的可食用且高度商业化的部分，因此在这项工作中，我们将重点放在叶子上，这是植物的一部分，被认为是农业工业废物。对辣椒根、茎和叶的水提取物生物合成金（AuNPs）和银（AgNPs）纳米颗粒进行了评价，用叶提取物获得了最佳结果。使用叶提取物合成的金和银纳米颗粒采用紫外-可见分光光度法（UV-Vis）、衰减全反射傅立叶变换红外光谱法（FTIR）对纳米金-叶和纳米银-叶进行了表征（FTIR-ATR）、X射线光电子能谱（XPS）、超高分辨率扫描电子显微镜与能量色散X射线能谱联用通过UHR-SEM-EDX和透射电镜（TEM）对产物进行了表征，并对其抗氧化活性和抗菌活性进行了测试。此外，还分析了参与纳米颗粒合成的不同代谢物。我们发现，通过使用来自叶子的提取物，我们可以产生稳定且易于合成的AuNPs和AgNPs。使用微波辐射合成AuNPs-叶，而使用UV光辐射合成AgNPs-叶。抗氧化活性的提取物，由ABTS测定，显示后的金纳米粒子叶和银纳米粒子叶的合成，分别下降了44.7%和60.7%。银纳米粒子-叶合成后，多酚，还原糖和氨基酸的浓度分别下降了15.4%，38.7%和46.8%，在叶提取物，而金纳米粒子-叶合成后，只有还原糖下降了67.7%。这些结果表明，这些分子组涉及纳米颗粒的还原/稳定。虽然这些化合物在金纳米粒子叶和银纳米粒子叶的合成中的贡献不同。最后，AgNPs-叶片抑制了S.金黄色葡萄球菌E. coli、S. marcescens和E.粪便所有这些都是具有临床重要性的细菌菌株，因为它们的抗生素耐药性发展迅速。
So far, several studies have focused on the synthesis of metallic nanoparticles making use of extracts from the fruit of the plants from the genus Capsicum. However, as the fruit is the edible, and highly commercial, part of the plant, in this work we focused on the leaves, a part of the plant that is considered agro-industrial waste. The biological synthesis of gold (AuNPs) and silver (AgNPs) nanoparticles using aqueous extracts of root, stem and leaf of Capsicum chinense was evaluated, obtaining the best results with the leaf extract. Gold and silver nanoparticles synthesized using leaf extract (AuNPs-leaf and AgNPs-leaf, respectively) were characterized by UV-visible spectrophotometry (UV-Vis), Fourier Transform Infrared Spectroscopy with Attenuated Total Reflection (FTIR-ATR), X-ray Photoelectron Spectroscopy (XPS), Ultra Hight Resolution Scanning Electron Microscopy coupled to Energy-Dispersive X-ray spectroscopy (UHR-SEM-EDX) and Transmission Electron Microscopy (TEM), and tested for their antioxidant and antimicrobial activities. In addition, different metabolites involved in the synthesis of nanoparticles were analyzed. We found that by the use of extracts derived from the leaf, we could generate stable and easy to synthesize AuNPs and AgNPs. The AuNPs-leaf were synthesized using microwave radiation, while the AgNPs-leaf were synthesized using UV light radiation. The antioxidant activity of the extract, determined by ABTS, showed a decrease of 44.7% and 60.7% after the synthesis of the AuNPs-leaf and AgNPs-leaf, respectively. After the AgNPs-leaf synthesis, the concentration of polyphenols, reducing sugars and amino acids decreased by 15.4%, 38.7% and 46.8% in the leaf extract, respectively, while after the AuNPs-leaf synthesis only reducing sugars decreased by 67.7%. These results suggest that these groups of molecules are implicated in the reduction/stabilization of the nanoparticles. Although the contribution of these compounds in the synthesis of the AuNPs-leaf and the AgNPs-leaf was different. Finally, the AgNPs-leaf inhibited the growth of S. aureus, E. coli, S. marcescens and E. faecalis. All of them are bacterial strains of clinical importance due to their fast antibiotic resistance development.
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1.8
作者：
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