Cerium Oxide Nanoparticles/Polyacrylonitrile Nanofibers as Impervious Barrier against Viral Infections.
Cerium Oxide Nanoparticles/Polyacrylonitrile Nanofibers as Impervious Barrier against Viral Infections.
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DOI：
10.3390/pharmaceutics15051494
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发表时间：
2023-05-13
期刊：
影响因子：
5.4
通讯作者：
Hathout RM
中科院分区：
文献类型：
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作者：
Emam MH;Elezaby RS;Swidan SA;Loutfy SA;Hathout RM
背景：佩戴口罩是降低冠状病毒传播率的防护措施之一。它的大规模传播需要使用纳米技术开发安全有效的抗病毒口罩（过滤器）。方法：将氧化铈纳米粒子（CeO2 NPs）掺入聚丙烯腈（PAN）静电纺纳米纤维中制备新型静电纺复合材料，该复合材料可用于未来的口罩。研究了聚合物浓度、外加电压和加料速率对静电纺丝的影响。采用SEM、XRD、FTIR和拉伸强度测试对电纺丝纳米纤维进行了表征。采用MTT比色法在Vero细胞系中评估了纳米纤维的细胞毒作用，并评估了纳米纤维对人腺病毒5型（ADV-5）呼吸道病毒的抗病毒活性。结果：最佳配方为PAN浓度为8%，w/v负载率为0.25%,w/v CeO2 NPs，加料速率为26 KV，施加电压为0.5 mL/h。它们的粒径为15.8±1.91 nm， zeta电位为- 14±0.141 mV。扫描电镜成像显示了纳米纤维的纳米级特征，即使加入了CeO2 NPs。细胞活力研究表明，聚丙烯腈纳米纤维是安全的。将CeO2 NPs加入到这些纤维中进一步提高了它们的细胞活力。此外，组装过滤器可以阻止病毒进入宿主细胞，并通过吸附和抗病毒机制阻止病毒在细胞内复制。结论：所制备的氧化铈纳米颗粒/聚丙烯腈纳米纤维是一种很有前途的抗病毒过滤器，可用于阻止病毒的传播。
Background: Using face masks is one of the protective measures to reduce the transmission rate of coronavirus. Its massive spread necessitates developing safe and effective antiviral masks (filters) applying nanotechnology. Methods: Novel electrospun composites were fabricated by incorporating cerium oxide nanoparticles (CeO2 NPs) into polyacrylonitrile (PAN) electrospun nanofibers that can be used in the future in face masks. The effects of the polymer concentration, applied voltage, and feeding rate during the electrospinning were studied. The electrospun nanofibers were characterized using SEM, XRD, FTIR, and tensile strength testing. The cytotoxic effect of the nanofibers was evaluated in the Vero cell line using the MTT colorimetric assay, and the antiviral activity of the proposed nanofibers was evaluated against the human adenovirus type 5 (ADV-5) respiratory virus. Results: The optimum formulation was fabricated with a PAN concentration of 8%, w/v loaded with 0.25%, w/v CeO2 NPs with a feeding rate of 26 KV and an applied voltage of 0.5 mL/h. They showed a particle size of 15.8 ± 1.91 nm and a zeta potential of −14 ± 0.141 mV. SEM imaging demonstrated the nanoscale features of the nanofibers even after incorporating CeO2 NPs. The cellular viability study showed the safety of the PAN nanofibers. Incorporating CeO2 NPs into these fibers further increased their cellular viability. Moreover, the assembled filter could prevent viral entry into the host cells as well as prevent their replication inside the cells via adsorption and virucidal antiviral mechanisms. Conclusions: The developed cerium oxide nanoparticles/polyacrylonitrile nanofibers can be considered a promising antiviral filter that can be used to halt virus spread.
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影响因子：
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作者：
Basak S;Packirisamy G
通讯作者：
Packirisamy G
影响因子：
5.8
作者：
Farid, Mariane M.;Hathout, Rania M.;Abou-Aisha, Khaled
通讯作者：
Abou-Aisha, Khaled
用于个人防护装备的增强抗菌和抗病毒活性的聚丙烯腈/聚四氢嘧啶（PAN/PTHP）纳米纤维的合成
影响因子：
13.6
作者：
Guo, Shuaibing;Yu, Bing;Shen, Youqing
通讯作者：
Shen, Youqing
影响因子：
5.8
作者：
Hathout, Rania M.;Gad, Heba A.;Metwally, Abdelkader A.
通讯作者：
Metwally, Abdelkader A.
DOI：
10.1007/978-1-4939-9516-5_6
发表时间：
2019-01-01
期刊：
PHARMACEUTICAL NANOTECHNOLOGY: BASIC PROTOCOLS
影响因子：
--
作者：
Hathout, Rania M.;Metwally, Abdelkader A.
通讯作者：
Metwally, Abdelkader A.