Ex vivo detection and quantification of apically extruded volatile compounds and disinfection by-products by SIFT-MS, during chemomechanical preparation of infected root canals.
Ex vivo detection and quantification of apically extruded volatile compounds and disinfection by-products by SIFT-MS, during chemomechanical preparation of infected root canals.
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在感染根管的化学机械制备过程中，通过 SIFT-MS 对根尖挤出的挥发性化合物和消毒副产物进行离体检测和定量。
DOI：
10.1016/j.dental.2019.11.011
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Ioannidis K
中科院分区：
文献类型：
--
作者：
Ioannidis K
ObjectivesTo assess the release and apical extrusion of toxic volatile compounds and disinfection by-products during instrumentation and irrigation of artificial infected root canal samples，with sodium hypochlorite and ethylene diamine tetraacetic. MethodsForty 2 single root human teeth decoronated to obtain 15 mm-long root samples and working length was determined 1 mm short of root apex.所有标本最初preflared，创造足够的锥形空间的营养强调多物种生物膜的发展。将标本随机分为三组[第1组;无牙髓干预，第2组;旋转锉器械和无菌生理盐水冲洗，第3组;旋转锉器械和2.5%次氯酸钠（NaOCl）和17%乙二胺四乙酸（EDTA）冲洗]。定制的实验模型装置制造的每个标本。将根尖根三分之一插入装有无菌超纯水的玻璃小瓶中，以模拟高顺应性根周间隙。通过选择离子流管质谱法（SIFT-MS）一式三份对从玻璃小瓶中获得的等分试样的反应产物进行真实的实时分析。采用双向方差分析（ANOVA）和事后Tukey检验进行数据分析。结果未进行根管治疗的患牙未出现挥发性化合物（VOCs）和消毒副产物（DBPs），而根管预备和冲洗（第2组和第3组）导致VOCs和DBPs从根尖排出。在第3组中，从根周间隙获得的等分试样释放高浓度的甲醇、丙醇、氨、氯仿，以及意外的更高水平的甲醛，与第2组相比具有统计学显著性（P < 0.05）。2.5%NaOCl和17%EDTA导致在水封闭的神经根周围空间中形成有毒VOC和DBP。NaOCl和EDTA的化学相互作用导致产生高浓度的甲醛。三氯甲烷和甲醛的生成表明，应进行潜在健康危害的风险评估。
ObjectivesTo assess the release and apical extrusion of toxic volatile compounds and disinfection by-products during instrumentation and irrigation of artificially infected root canal specimens, with sodium hypochlorite and ethylene diamine tetra acetic acid.MethodsForty-two single-rooted human teeth were decoronated to obtain 15 mm-long root specimens and working length was determined 1 mm short of root apex. All specimens were initially preflared, to create sufficient conical space for the development of a nutrient-stressed multispecies biofilm. The specimens were randomly assigned into three groups [Group 1; no endodontic intervention, Group 2; instrumentation with rotary files and irrigation with sterile saline, Group 3; instrumentation with rotary files and irrigation with 2.5% sodium hypochlorite (NaOCl) and 17% ethylene diamine tetra acetic acid (EDTA)]. A customised experimental model apparatus was fabricated for each specimen. The apical root third was inserted in a glass vial filled with sterile ultrapure water, to simulate high-compliance periradicular space. The reaction products of the aliquots obtained from the glass vials were analysed in real time, by selected ion flow tube mass spectrometry (SIFT-MS) in triplicates. Two-way analysis of variance (ANOVA) with post hoc Tukey tests were used for data analysis. The level of statistical significance was set at P < 0.05.ResultsThe group of teeth that were not subjected to endodontic intervention did not show any volatile compounds (VOCs) or disinfection by products (DBPs) whilst instrumentation and irrigation of root canals (Groups 2 and 3) resulted in the apical extrusion of VOCs and DBPs. In Group 3, the aliquots obtained from periradicular space released high concentrations of methanol, propanol, ammonia, chloroform, together with unexpected higher levels of formaldehyde, which were statistically significant compared to Group 2 (P < 0.05).SignificanceThe mechanical preparation and irrigation of artificially infected root canals with rotary files, 2.5% NaOCl and 17% EDTA resulted in the formation of toxic VOCs and DBPs in a water-closed periradicular space. The chemical interaction of NaOCl and EDTA resulted in the generation of high concentrations of formaldehyde. The formation of chloroform and formaldehyde indicate that risk assessment of the potential hazards to health should be carried out.
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DOI：
10.1016/b978-0-12-386454-3.00344-4
发表时间：
2020
期刊：
Federal Regulatory Guide
影响因子：
--
作者：
通讯作者：
--
DOI：
10.3200/aeoh.60.1.7-15
发表时间：
2005-01-01
期刊：
ARCHIVES OF ENVIRONMENTAL HEALTH
影响因子：
--
作者：
Ashley, David L.;Blount, Benjamin C.;Masters, John
通讯作者：
Masters, John
影响因子：
--
作者：
Al Shahrani, Mohammed;DiVito, Enrico;Huang, George T. -J.
通讯作者：
Huang, George T. -J.
影响因子：
4.2
作者：
Prado, Maira;Santos, Helvecio M., Jr.;Gomes, Brenda P. F. A.
通讯作者：
Gomes, Brenda P. F. A.
影响因子：
5
作者：
Villanueva, Cristina M.;Cantor, Kenneth P.;Kogevinas, Manolis
通讯作者：
Kogevinas, Manolis