Comprehensive proteomic quantification of bladder stone progression in a cystinuric mouse model using data-independent acquisitions.
Comprehensive proteomic quantification of bladder stone progression in a cystinuric mouse model using data-independent acquisitions.
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DOI：
10.1371/journal.pone.0250137
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
胱氨酸尿症是引起泌尿系统生物矿化的各种疾病之一，包括人类膀胱结石形成。它在儿童和青少年中最普遍，在男性中更具侵略性。没有治愈方法，只有有限的疾病管理技术有助于溶解结石。即使在治疗后，复发也经常发生。除了胱氨酸的积累，很少有人知道这些结石的形成，扩张和复发的因素。本研究旨在通过微计算机断层扫描成像来确定膀胱结石的生长，并在胱氨酸尿症小鼠模型中描述动态结石蛋白质组变化。膀胱结石在体内发育后，根据其大小将其收获并分为四个发育阶段（砂，小，中，大结石）。数据依赖和数据独立的采集允许深入分析石头蛋白质组学。蛋白质组特征和途径说明了随着结石生长的主要变化。结石从小病灶开始，向外生长，并显示出核糖体蛋白和凝血和血小板脱粒相关因子的主要富集，表明特定途径的主要失调，可以靶向新的治疗选择。
Cystinuria is one of various disorders that cause biomineralization in the urinary system, including bladder stone formation in humans. It is most prevalent in children and adolescents and more aggressive in males. There is no cure, and only limited disease management techniques help to solubilize the stones. Recurrence, even after treatment, occurs frequently. Other than a buildup of cystine, little is known about factors involved in the formation, expansion, and recurrence of these stones. This study sought to define the growth of bladder stones, guided by micro-computed tomography imaging, and to profile dynamic stone proteome changes in a cystinuria mouse model. After bladder stones developed in vivo, they were harvested and separated into four developmental stages (sand, small, medium and large stone), based on their size. Data-dependent and data-independent acquisitions allowed deep profiling of stone proteomics. The proteomic signatures and pathways illustrated major changes as the stones grew. Stones initiate from a small nidus, grow outward, and show major enrichment in ribosomal proteins and factors related to coagulation and platelet degranulation, suggesting a major dysregulation in specific pathways that can be targeted for new therapeutic options.
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影响因子：
4.4
作者：
Bourderioux, Matthieu;Thao Nguyen-Khoa;Guerrera, Ida Chiara
通讯作者：
Guerrera, Ida Chiara
影响因子：
--
作者：
Canales, Benjamin K.;Anderson, Lorraine;Monga, Manoj
通讯作者：
Monga, Manoj
影响因子：
2.1
作者：
Jou, Yeong-Chin;Fang, Chiung-Yao;Tsai, Yuh-Shyan
通讯作者：
Tsai, Yuh-Shyan
影响因子：
3.5
作者：
Liu, Jian-dong;Liu, Jian-jun;Zhuang, Zhi-xiong
通讯作者：
Zhuang, Zhi-xiong
DOI：
10.1097/01.asn.0000029586.17680.e5
发表时间：
2002-10-01
影响因子：
13.6
作者：
Dello Strologo, L;Pras, E;Rizzoni, G
通讯作者：
Rizzoni, G