Preliminary antimycobacterial study on selected Turkish plants (Lamiaceae) against Mycobacterium tuberculosis and search for some phenolic constituents.
Preliminary antimycobacterial study on selected Turkish plants (Lamiaceae) against Mycobacterium tuberculosis and search for some phenolic constituents.
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DOI：
10.1186/1472-6882-13-365
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发表时间：
2013-12-21
影响因子：
--
通讯作者：
Aydeniz H
中科院分区：
文献类型：
--
作者：
Askun T;Tekwu EM;Satil F;Modanlioglu S;Aydeniz H
结核病在全球的死灰复燃是一个重大威胁。唇形科成员几个世纪以来一直用于民间疗法。本研究旨在评估六种植物（唇形科）十八种粗提物的体外抗分枝杆菌活性，并表征其酚类和类黄酮化合物。土耳其唇形科六种药用植物（Stachys tmolea Boiss.、Stachys thirkei C. Koch、Ballota acetabulosa (L.) Benth.、Thymus sipthorpii Benth.、Satureja aintabensis P.H. Davis 和 Micromeria juliana (L.) Benth. ex Reich.）于 2009 年至 2010 年采集。粉碎的植物样品按照极性递增的顺序依次用石油醚、乙酸乙酯和甲醇进行萃取。采用肉汤微量稀释法筛选针对四种结核分枝杆菌菌株的提取物。通过液相色谱-质谱法对酚类和黄酮类化合物进行了表征。发现 S. aintabensis、T. sibthorpii 和 M. juliana 对四种结核分枝杆菌菌株具有相当大的活性，最小抑制浓度值为 12.5-100 μg/ml。 S. aintabensis 和 T. sibthorpii 提取物可杀死结核分枝杆菌，最低杀菌浓度值为 50–800 μg/ml。基于这些显着的抗分枝杆菌活性，我们认为它们可能是天然抗结核药物的来源。 S. aintabensis 和 T. sibthorpii 通过杀死分枝杆菌菌株表现出活性。 T. sibthorpii 和 S. aintabensis 的主要酚类化合物是迷迭香。类黄酮可能是药物设计的“模式”。
The global resurgence of tuberculosis is a significant threat. Lamiaceae members have been used in folk remedies for centuries. This study was designed to assess the in-vitro antimycobacterial activity of eighteen crude extracts from six plants (Lamiaceae) and to characterize their phenolic and flavonoid compounds. Six Turkish medicinal plants of the family Lamiaceae (Stachys tmolea Boiss., Stachys thirkei C. Koch, Ballota acetabulosa (L.) Benth., Thymus sipthorpii Benth., Satureja aintabensis P.H. Davis, and Micromeria juliana (L.) Benth. ex Reich.) were collected in 2009 – 2010. Dried and crushed plant samples were subjected to sequential extraction with petroleum ether, ethyl acetate, and methanol in order of increasing polarity. A broth microdilution method was employed to screen extracts against four mycobacterial strains of Mycobacterium tuberculosis. Phenolic and flavonoid compounds were characterized by liquid chromatography–mass spectrometry. S. aintabensis, T. sibthorpii, and M. juliana were found to develop considerable activity against the four strains of M. tuberculosis with the minimal inhibitory concentrations value of 12.5-100 μg/ml. S. aintabensis and T. sibthorpii extracts killed M. tuberculosis with the minimum bactericidal concentration value of 50–800 μg/ml. On the basis of these prominent antimycobacterial activity, we suggest that they could be a source of natural anti-tuberculosis agents. S. aintabensis and T. sibthorpii showed activity by killing Mycobacteria strains. The major phenolic compound was rosmarinic for T. sibthorpii and S. aintabensis. Flavonoids might be “a modal” for the drug design.
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影响因子：
7.2
作者：
Jimenez-Arellanes, A;Meckes, M;Luna-Herrera, J
通讯作者：
Luna-Herrera, J
影响因子：
3.8
作者：
Askun, T.;Tumen, G.;Kilic, T.
通讯作者：
Kilic, T.
影响因子：
3.8
作者：
Askun, Tulin;Tumen, Gulendam;Ates, Mustafa
通讯作者：
Ates, Mustafa
影响因子：
7.2
作者：
Gordien, Andrea Y.;Gray, Alexander I.;Seidel, Veronique
通讯作者：
Seidel, Veronique
影响因子：
5.4
作者：
Karaman, S;Digrak, M;Ilcim, A
通讯作者：
Ilcim, A