Protective functions of alternative splicing transcripts (CdDHN4-L and CdDHN4-S) of CdDHN4 from bermudagrass under multiple abiotic stresses
Protective functions of alternative splicing transcripts (CdDHN4-L and CdDHN4-S) of CdDHN4 from bermudagrass under multiple abiotic stresses
复制标题
多种非生物胁迫下狗牙根 CdDHN4 选择性剪接转录本 (CdDHN4-L 和 CdDHN4-S) 的保护功能
DOI：
10.1016/j.gene.2020.100033
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发表时间：
2020-04
期刊：
影响因子：
--
通讯作者：
Peng Zhou
中科院分区：
文献类型：
--
作者：
Di Zhang;Aimin Lv;Tianchen Yang;Xiaoqing Cheng;Enhua Zhao;Peng Zhou
脱氢酶在植物对非生物胁迫的适应中起着重要作用。本研究的目的是表征在狗牙根（狗牙根属）的DHN。从狗牙根‘Tifway’中克隆了CdDHN 4基因。CdDHN 4-S和CdDHN 4-L蛋白均为YSK 2型DHN，其中CdDHN 4-L中存在Φ片段，CdDHN 4-S中不存在Φ片段。与野生型相比，表达CdDHN 4-LorCdDHN 4-S的转基因拟南芥表现出对盐、渗透压、低温和干旱胁迫的耐受性提高。两个转基因株系在耐盐性和耐旱性上没有差异，但表达CdDHN 4-S的植株在渗透胁迫下比表达CdDHN 4-L的植株生长更好。在盐胁迫和干旱胁迫下，两个转基因株系的丙二醛（MDA）和活性氧（ROS）含量均低于野生型，抗氧化酶活性高于野生型，其中CdDHN 4-S的ROS清除能力高于CdDHN 4-L。
Dehydrins (DHNs) play critical roles in plant adaptation to abiotic stresses. The objective of this study was to characterize DHNs in bermudagrass (Cynodonspp.).CdDHN4gene was cloned from bermudagrass ‘Tifway’. TwoCdDHN4transcripts were detected due to alternative splicing (the nonsplicedCdDHN4-Land the splicedCdDHN4-S) and both the CdDHN4-S and CdDHN4-L proteins are YSK2-type DHNs, the Φ-segment is present in CdDHN4-L and absent in CdDHN4-S. TransgenicArabidopsis thalianaexpressingCdDHN4-LorCdDHN4-Sexhibited improved tolerance to salt, osmotic, low temperature and drought stress compared to the wild type (WT). The two transgenic lines did not differ in salt or drought tolerance, while plants expressingCdDHN4-Sgrew better under osmotic stress than those expressingCdDHN4-L. Both transgenic lines exhibited reduced content of malondialdehyde (MDA) and reactive oxygen species (ROS); and higher antioxidant enzymatic activities than the wild type plants under salt or drought stress.CdDHN4-Sexhibited a higher ROS-scavenging capacity thanCdDHN4-L.
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影响因子：
4.2
作者：
Hill, William;Jin, Xiao-Lu;Zhang, Xing-Hai
通讯作者：
Zhang, Xing-Hai
脱水蛋白、CdDHN4-L 和 CdDHN4-S 的表征及其体外对抗非生物胁迫的不同保护作用
影响因子：
5.3
作者：
Lv A;Su L;Liu X;Xing Q;Huang B;An Y;Zhou P
通讯作者：
Zhou P
影响因子：
3.7
作者：
Kalaji, Hazem M.;Schansker, Gert;Ladle, Richard J.;Goltsev, Vasilij;Bosa, Karolina;Allakhverdiev, Suleyman I.;Brestic, Marian;Bussotti, Filippo;Calatayud, Angeles;Dabrowski, Piotr;Elsheery, Nabil I.;Ferroni, Lorenzo;Guidi, Lucia;Hogewoning, Sander W.;Jajoo, Anjana;Misra, Amarendra N.;Nebauer, Sergio G.;Pancaldi, Simonetta;Penella, Consuelo;Poli, DorothyBelle;Pollastrini, Martina;Romanowska-Duda, Zdzislawa B.;Rutkowska, Beata;Serodio, Joao;Suresh, Kancherla;Szulc, Wieslaw;Tambussi, Eduardo;Yanniccari, Marcos;Zivcak, Marek
通讯作者：
Zivcak, Marek
影响因子：
5.1
作者：
LANG, V;PALVA, ET
通讯作者：
PALVA, ET
影响因子：
4.6
作者：
Liu Y;Wang L;Zhang T;Yang X;Li D
通讯作者：
Li D