Genomic screening of 16 UK native bat species through conservationist networks uncovers coronaviruses with zoonotic potential.
Genomic screening of 16 UK native bat species through conservationist networks uncovers coronaviruses with zoonotic potential.
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DOI：
10.1038/s41467-023-38717-w
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发表时间：
2023-06-27
影响因子：
16.6
通讯作者：
Savolainen, Vincent
中科院分区：
文献类型：
--
作者：
Tan, Cedric C. S.;Trew, Jahcub;Peacock, Thomas P.;Mok, Kai Yi;Hart, Charlie;Lau, Kelvin;Ni, Dongchun;Orme, C. David L.;Ransome, Emma;Pearse, William D.;Coleman, Christopher M.;Bailey, Dalan;Thakur, Nazia;Quantrill, Jessica L.;Sukhova, Ksenia;Richard, Damien;Kahane, Laura;Woodward, Guy;Bell, Thomas;Worledge, Lisa;Nunez-Mino, Joe;Barclay, Wendy;van Dorp, Lucy;Balloux, Francois;Savolainen, Vincent
欧洲对蝙蝠传播的冠状病毒的特征描述有限。在这里，我们从英国17种蝙蝠中的16种中筛选了48种粪便样本中的冠状病毒，这些样本是通过蝙蝠康复和保护主义者网络收集的。我们在六种蝙蝠物种中恢复了九个完整的基因组，包括两种新型冠状病毒：四种冠状病毒，一种MERS相关的β冠状病毒和四种密切相关的肉瘤病毒。我们证明，这些肉瘤病毒中至少有一种可以结合并利用人类ACE 2受体感染人类细胞，尽管效果不佳。此外，这些Sarbecoviruses的刺突蛋白具有R-A-K-Q基序，该基序距离弗林蛋白酶切割位点（FCS）仅一个核苷酸突变，该位点增强了其他冠状病毒（包括SARS-CoV-2）的感染性。然而，将该基序突变为FCS不能实现刺突切割。总的来说，虽然英国肉瘤病毒需要进一步的分子适应才能感染人类，但它们的人畜共患病风险需要更密切的监测。某些蝙蝠物种先前已被确定为感染人类的冠状病毒的祖先来源，但关于英国感染蝙蝠的病毒的基因组多样性或人畜共患病潜力的数据有限。在这里，作者使用深度测序和体外分析来检测从48个蝙蝠粪便样本中回收的冠状病毒。
There has been limited characterisation of bat-borne coronaviruses in Europe. Here, we screened for coronaviruses in 48 faecal samples from 16 of the 17 bat species breeding in the UK, collected through a bat rehabilitation and conservationist network. We recovered nine complete genomes, including two novel coronavirus species, across six bat species: four alphacoronaviruses, a MERS-related betacoronavirus, and four closely related sarbecoviruses. We demonstrate that at least one of these sarbecoviruses can bind and use the human ACE2 receptor for infecting human cells, albeit suboptimally. Additionally, the spike proteins of these sarbecoviruses possess an R-A-K-Q motif, which lies only one nucleotide mutation away from a furin cleavage site (FCS) that enhances infectivity in other coronaviruses, including SARS-CoV-2. However, mutating this motif to an FCS does not enable spike cleavage. Overall, while UK sarbecoviruses would require further molecular adaptations to infect humans, their zoonotic risk warrants closer surveillance. Certain bats species have previously been identified as ancestral sources of coronaviruses that infect humans but there is limited data on the genomic diversity or zoonotic potential of viruses infecting bats in the UK. Here, the authors use deep sequencing and in vitro assays to characterise coronaviruses recovered from 48 bat faecal samples.
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影响因子：
14.9
作者：
Blum M;Chang HY;Chuguransky S;Grego T;Kandasaamy S;Mitchell A;Nuka G;Paysan-Lafosse T;Qureshi M;Raj S;Richardson L;Salazar GA;Williams L;Bork P;Bridge A;Gough J;Haft DH;Letunic I;Marchler-Bauer A;Mi H;Natale DA;Necci M;Orengo CA;Pandurangan AP;Rivoire C;Sigrist CJA;Sillitoe I;Thanki N;Thomas PD;Tosatto SCE;Wu CH;Bateman A;Finn RD
通讯作者：
Finn RD
DOI：
10.3390/v13040599
发表时间：
2021-04-01
期刊：
Viruses
影响因子：
--
作者：
Holbrook MG;Anthony SJ;Navarrete-Macias I;Bestebroer T;Munster VJ;van Doremalen N
通讯作者：
van Doremalen N
影响因子：
64.8
作者：
Eby, Peggy;Peel, Alison J.;Hoegh, Andrew;Madden, Wyatt;Giles, John R.;Hudson, Peter J.;Plowright, Raina K.
通讯作者：
Plowright, Raina K.
影响因子：
4.6
作者：
Crook JM;Murphy I;Carter DP;Pullan ST;Carroll M;Vipond R;Cunningham AA;Bell D
通讯作者：
Bell D
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D