Modulation of plant architecture by the miR156f-OsSPL7-OsGH3.8 pathway in rice.
Modulation of plant architecture by the miR156f-OsSPL7-OsGH3.8 pathway in rice.
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DOI：
10.1093/jxb/ery273
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发表时间：
2018-10-12
影响因子：
6.9
通讯作者：
Shi Z
中科院分区：
文献类型：
--
作者：
Dai Z;Wang J;Yang X;Lu H;Miao X;Shi Z
miR156f通过其靶点OsSPL7直接结合OsGH3.8启动子来调节水稻植株结构，从而允许与生长素信号通路进行串扰。分蘖数和株高是直接影响水稻产量的两个主要植株结构特征。生长素和miR156（一种被广泛研究的小RNA）都广泛参与植物的发育和生理，这表明两者之间可能存在联系。在本研究中，我们鉴定了一个水稻T-DNA插入簇和矮化（cd）突变体，该突变体分蘖数增加，株高降低。T-DNA插入与miR156f基因非常接近，并与其上调有关。过表达miR156f的植株与cd突变体相似。相比之下，过表达miR156f靶模拟物（MIM156fOE）的植株分蘖数减少，株高增加。遗传分析表明OsSPL7是miR156f调控植物结构的靶点。过表达OsSPL7的植株分蘖数减少，而OsSPL7 RNAi植株分蘖数增加，高度降低。我们还发现OsSPL7直接与OsGH3.8启动子结合以调节其转录。过表达OsGH3.8和OsGH3.8 RNAi分别部分补充了MIM156fOE和cd突变型。我们的综合数据表明，miR156f-OsSPL7-OsGH3.8通路调节水稻分蘖数和株高，该通路可能允许miR156与生长素之间的串扰。
miR156f modulates rice plant architecture by direct binding to the OsGH3.8 promoter through its target OsSPL7, to allow crosstalk with the auxin signaling pathway. Tiller number and plant height are two of the main features of plant architecture that directly influence rice yield. Auxin and miR156, an extensively studied small RNA (smRNA), are both broadly involved in plant development and physiology, suggesting a possible relationship between the two. In this study, we identified a rice T-DNA insertion cluster and dwarf (cd) mutant that has an increased tiller number and reduced plant height. The T-DNA insertion was in close proximity to the miR156f gene and was associated with its up-regulation. Plants overexpressing miR156f resembled the cd mutant. In contrast, plants overexpressing an miR156f target mimic (MIM156fOE) had a reduced tiller number and increased height. Genetic analysis showed that OsSPL7 is a target of miR156f that regulates plant architecture. Plants overexpressing OsSPL7 had a reduced tiller number, while OsSPL7 RNAi plants had an increased tiller number and a reduced height. We also found that OsSPL7 binds directly to the OsGH3.8 promoter to regulate its transcription. Overexpression of OsGH3.8 and OsGH3.8 RNAi partially complemented the MIM156fOE and cd mutant phenotypes, respectively. Our combined data show that the miR156f–OsSPL7–OsGH3.8 pathway regulates tiller number and plant height in rice, and this pathway may allow crosstalk between miR156 and auxin.
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影响因子：
4.6
作者：
Hayashi-Tsugane M;Maekawa M;Tsugane K
通讯作者：
Tsugane K
影响因子：
11.6
作者：
Cho, Sung Hyun;Coruh, Ceyda;Axtell, Michael J.
通讯作者：
Axtell, Michael J.
DOI：
10.1007/978-1-60761-702-0_15
发表时间：
2010-01-01
期刊：
PLANT STRESS TOLERANCE: METHODS AND PROTOCOLS
影响因子：
--
作者：
Jia, Xiaoyun;Mendu, Venugopal;Tang, Guiliang
通讯作者：
Tang, Guiliang
影响因子：
7.2
作者：
Cui, Long-Gang;Shan, Jun-Xiang;Lin, Hong-Xuan
通讯作者：
Lin, Hong-Xuan
影响因子：
5.1
作者：
Hellens RP;Allan AC;Friel EN;Bolitho K;Grafton K;Templeton MD;Karunairetnam S;Gleave AP;Laing WA
通讯作者：
Laing WA