Genetic analysis of the Gdh and Bg genes of animal-derived Giardia duodenalis isolates in Northeastern China and evaluation of zoonotic transmission potential.
Genetic analysis of the Gdh and Bg genes of animal-derived Giardia duodenalis isolates in Northeastern China and evaluation of zoonotic transmission potential.
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DOI：
10.1371/journal.pone.0095291
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Cao J
中科院分区：
文献类型：
--
作者：
Liu A;Yang F;Shen Y;Zhang W;Wang R;Zhao W;Zhang L;Ling H;Cao J
十二指肠贾第虫是一种常见的肠道寄生虫，感染人类和许多其他哺乳动物，主要分布在一些卫生条件差的地区。在不同的地理区域，由动物源性十二指肠贾第虫引起的人类贾第虫病负担所占比例不同。在中国大陆，来自动物的十二指肠棘球蚴gdh和bg基因的遗传数据仅局限于狗和猫。本研究的目的是提供中国东北黑龙江省两个基因座动物源性十二指肠螺旋体分离株（兔、羊和牛）的遗传特征信息，并评估其人畜共患传播的可能性。本研究从黑龙江省动物粪便（奶牛、肉牛、绵羊和家兔）中分离得到61株十二指肠双歧杆菌，经gdh和bg位点鉴定。羊源性十二指肠螺杆菌组合AI的gdh和bg基因序列与人源性十二指肠螺杆菌组合B的gdh基因序列具有100%的相似性。结果表明，在gdh位点上分别鉴定出1个和7个组合A和E亚型，在bg位点上分别鉴定出2个和3个组合B和E亚型。在羊和牛中发现了3对相同的组合E的bg序列。本文首次报道了中国大陆家兔、绵羊和牛十二指肠棘球蚴gdh和bg基因的遗传特征。AI和B的同源性分析提示人畜共患传播的可能性。十二指肠巨噬菌的新亚型组合可能代表了中国黑龙江省十二指肠巨噬菌的特有遗传特征。
Giardia duodenalis is a common intestinal parasite that infects humans and many other mammals, mainly distributing in some areas with poor sanitation. The proportion of the human giardiasis burden attributable to G. duodenalis of animal origin differs in different geographical areas. In Mainland China, genetic data of the gdh and bg genes of G. duodenalis from animals are only limited in dogs and cats. The aim of the study was to provide information on the genetic characterizations of animal-derived G. duodenalis isolates (from rabbits, sheep and cattle) at both loci in Heilongjiang Province, Northeastern China, and to assess the potential for zoonotic transmission. 61 G. duodenalis isolates from animal feces (dairy and beef cattle, sheep and rabbits) in Heilongjiang Province were characterized at the gdh and bg loci in the present study. The gdh and bg gene sequences of sheep-derived G. duodenalis assemblage AI, and the gdh sequences of rabbit-derived G. duodenalis assemblage B had 100% similarity with those from humans, respectively. Novel subtypes of G. duodenalis were identified, with one and seven subtypes for assemblages A and E at the gdh locus, and two and three subtypes for assemblages B and E at the bg locus, respectively. Three pairs of the same bg sequences of assemblage E were observed in sheep and cattle. This is the first description of genetic characterizations of the gdh and bg genes of G. duodenalis from rabbits, sheep and cattle in Mainland China. Homology analysis of assemblages AI and B implied the possibility of zoonotic transmission. The novel subtypes of assemblages of G. duodenalis may represent the endemic genetic characteristics of G. duodenalis in Heilongjiang Province, China.
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