Presence and molecular characterisation of Giardia and Cryptosporidium in alpacas (Vicugna pacos) from Peru.
Presence and molecular characterisation of Giardia and Cryptosporidium in alpacas (Vicugna pacos) from Peru.
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DOI：
10.1016/j.vetpar.2012.01.025
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发表时间：
2012-07-06
影响因子：
2.6
通讯作者：
Pedraza-Díaz S
中科院分区：
文献类型：
--
作者：
Gómez-Couso H;Ortega-Mora LM;Aguado-Martínez A;Rosadio-Alcántara R;Maturrano-Hernández L;Luna-Espinoza L;Zanabria-Huisa V;Pedraza-Díaz S
通过免疫荧光显微镜和PCR扩增贾第鞭毛虫ssu-rRNA和β-贾第鞭毛虫素基因片段并测序，对来自秘鲁12个羊驼群的274份羊驼粪便样品中贾第鞭毛虫和隐孢子虫的存在进行了研究。以及来自隐孢子虫属（Cryptosporidium spp.）共137份样本（50.0%）对贾第虫属呈阳性，隐孢子虫12份，占4.4%。在10个（3.6%）样本中发现两种病原体的混合感染，发现贾第虫和隐孢子虫的群体感染率分别为91.7%（11/12群）和58.3%（7/12群）。关于动物的年龄，虽然在1周龄的动物中检测到贾第虫，但患病率随着年龄的增长而增加，到8周龄时达到80%。同样，在8周龄组中也发现隐孢子虫检出率最高（20%）。通过PCR，274个分析样本中有92个对贾第虫呈阳性。扩增产物测序结果表明，67份样品中存在贾第鞭毛虫组合A;在24个样品中的pesticalis组合E;和不一致的结果之间的两个分子标记在进一步的样品中使用。在274份样本中，仅在3份样本中通过PCR检测到隐孢子虫;在2份样本中鉴定出微小隐孢子虫，在1份样本中鉴定出隐孢子虫。本研究是首次对秘鲁羊驼中的两种寄生虫进行分子鉴定，也是首次报道C。无处不在的宿主对G. A、C. parvum和C.普遍存在，表明这些肠道病原体在羊驼和人类之间的人畜共患病传播是可能的。
The presence of Giardia and Cryptosporidium was investigated in 274 faecal samples of alpacas (Vicugna pacos) from 12 herds from Peru by immunofluorescence microscopy and PCR amplification and sequencing of fragments of the ssu-rRNA and β-giardin genes from Giardia spp., as well as the ssu-rRNA gene from Cryptosporidium spp. A total of 137 samples (50.0%) were positive for Giardia spp., and 12 samples (4.4%) for Cryptosporidium spp. In ten samples (3.6%), co-infection by both pathogens was found. Herd prevalence was found to be 91.7% (11/12 herds) for Giardia and 58.3% (7/12 herds) for Cryptosporidium. Regarding the age of the animals, although Giardia was detected in animals as young as 1 week, the prevalence increased with age, reaching 80% by 8 weeks. Similarly, the highest percentage of Cryptosporidium detection (20%) was also found in the 8 week-old group. By PCR, 92 of the 274 analysed samples were positive for Giardia. Sequencing of the amplicons showed the existence of Giardia duodenalis assemblage A in 67 samples; G. duodenalis assemblage E in 24 samples; and inconsistent results between the two molecular markers used in a further sample. Cryptosporidium was only detected by PCR in 3 of the 274 samples; Cryptosporidium parvum was identified in two samples and Cryptosporidium ubiquitum in one sample. This study is the first performing molecular characterisation of both parasites in Peruvian alpacas, and the first report of C. ubiquitum in this host. The identification of G. duodenalis assemblage A, C. parvum and C. ubiquitum, suggests that zoonotic transmission of these enteropathogens between alpacas and humans is possible.
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影响因子：
2.6
作者：
Appelbee, AJ;Frederick, LM;Olson, ME
通讯作者：
Olson, ME
DOI：
10.1016/j.tvjl.2007.09.022
发表时间：
2008-07
期刊：
Veterinary journal (London, England : 1997)
影响因子：
--
作者：
Thompson RC;Palmer CS;O'Handley R
通讯作者：
O'Handley R
影响因子：
2.2
作者：
Ey, PL;Mansouri, M;Mayrhofer, G
通讯作者：
Mayrhofer, G
DOI：
10.2460/javma.2003.223.1806
发表时间：
2003-12-15
期刊：
JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
影响因子：
--
作者：
Cebra, CK;Mattson, DE;Dearing, PL
通讯作者：
Dearing, PL
影响因子：
4.4
作者：
Ryan, U;Xiao, LH;Pavlasek, I
通讯作者：
Pavlasek, I