Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells.
Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells.
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DOI：
10.3343/kjlm.2011.31.3.212
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发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
Song KE
中科院分区：
文献类型：
--
作者：
Cho HS;Song IH;Park SY;Sung MC;Ahn MW;Song KE
本研究的目的是探讨生长因子（GFs）浓度的个体差异是否影响富血小板血浆（PRP）对人间充质干细胞（HMSCs）的生物学效应。采用ELISA法测定了活化PRP（aPRP）中7种代表性GF的浓度。使用来自3个供体的几种浓度的aPRP检查PRP对HMSCs的增殖和碱性磷酸酶（ALP）活性的影响;然后使用来自39个供体的5个亚组的10%aPRP评价GF水平与这些生物学效应之间的关系。将HMSCs在添加aPRP的DMEM中培养4或12天，然后测定DNA含量和ALP活性。当aPRP浓度为10%时，DNA含量显著增加，但ALP活性在此浓度下受到抑制。GF浓度在供体之间变化，并且通过聚类分析确定了特征性GF释放模式的5个亚组。DNA水平在组间存在显著差异，并且在转化生长因子-β 1（TGF-β1）和血小板衍生生长因子（PDGF）浓度较高的组中往往较高。DNA含量与TGF-β1浓度呈正相关，与供者年龄呈负相关。ALP活性与PDGF-BB浓度呈负相关。不同的GF浓度可能导致不同的生物学效应;因此，GF水平的个体差异应被考虑用于可靠的生物学功能解释和PRP的标准化应用。
The objective of this study was to explore whether individual variations in the concentration of growth factors (GFs) influence the biologic effects of platelet-rich plasma (PRP) on human mesenchymal stem cells (HMSCs). The concentrations of 7 representative GFs in activated PRP (aPRP) were measured using ELISA. The effects of PRP on the proliferation and alkaline phosphatase (ALP) activity of HMSCs were examined using several concentrations of aPRP from 3 donors; the relationships between the GF levels and these biologic effects were then evaluated using 10% aPRP from 5 subgroups derived from 39 total donors. HMSCs were cultured in DMEM with the addition of aPRP for 4 or 12 days; then, DNA content and ALP activity were measured. The quantity of DNA increased significantly at a 10% concentration of aPRP, but the ALP activity was suppressed at this concentration of aPRP. The GF concentrations varied among donors, and 5 subgroups of characteristic GF release patterns were identified via cluster analysis. DNA levels differed significantly between groups and tended to be higher in groups with higher concentrations of transforming growth factor-beta1 (TGF-β1) and platelet-derived growth factors (PDGFs). DNA quantity was positively correlated with TGF-β1 concentration, and was negatively correlated with donor age. ALP activity was negatively correlated with PDGF-BB concentration. The varying GF concentrations may result in different biologic effects; thus, individual differences in GF levels should be considered for reliable interpretation of the biologic functions and standardized application of PRP.
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DOI：
10.1007/s12306-008-0064-1
发表时间：
2008-12-01
期刊：
La Chirurgia degli organi di movimento
影响因子：
--
作者：
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影响因子：
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DOI：
10.1097/01.blo.0000165736.87628.12
发表时间：
2005-06-01
影响因子：
4.2
作者：
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通讯作者：
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影响因子：
3.3
作者：
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