Immunolocalization of matrix metalloproteinases and TIMP-1 (tissue inhibitor of metalloproteinases) in human gingival tissues from periodontitis patients.
Immunolocalization of matrix metalloproteinases and TIMP-1 (tissue inhibitor of metalloproteinases) in human gingival tissues from periodontitis patients.
复制标题
牙周炎患者牙龈组织中基质金属蛋白酶和 TIMP-1（金属蛋白酶组织抑制剂）的免疫定位。
DOI：
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发表时间：
1994
影响因子：
3.5
通讯作者：
J. Reynolds
中科院分区：
文献类型：
--
作者：
M. Meikle;R. Hembry;J. Holley;C. Horton;C. G. McFarlane;J. Reynolds
基质金属蛋白酶（MMPs）胶原酶，明胶酶A（72 kDa明胶酶），基质溶解素，及其特异性抑制剂TIMP-1（金属蛋白酶的组织抑制剂），使用特异性多克隆抗血清免疫定位在牙龈组织从21例慢性炎症性牙周病。还使用抗巨噬细胞（Leu-M5）、B细胞（Leu-14）、辅助性T细胞（OKT 4）、抑制性T细胞（OKT 8）和HLA-DR表位的单克隆抗体来鉴定白细胞亚群。在组织学显示重塑迹象的部位的结缔组织中观察到MMP。然而，阳性细胞的数量和分布变化很大，不仅在个体活检标本之间，而且在同一标本内。炎性细胞浸润的成分和分布也是如此。此外，尽管在一些标本中MMP产生细胞的数量与炎症的严重程度之间存在正相关性，但对于具有可比白细胞亚群评分的其他标本，数量减少，有时完全不存在。分泌MMPs的细胞为成纤维细胞、巨噬细胞和上皮细胞。不可能明确确定结缔组织内的MMP阳性细胞是成纤维细胞还是巨噬细胞，因为抗血清识别成纤维细胞和巨噬细胞MMP，并且MMP（4%多聚甲醛）和Leu-M5（丙酮）的不同固定要求排除了同一切片上的共定位。TIMP-1免疫定位于组织重塑部位的结缔组织细胞内。我们的研究结果支持这一假设，即组织来源的基质金属蛋白酶可能参与牙周病的组织重塑，并最终证明，上皮细胞可能参与以及结缔组织细胞。
The matrix metalloproteinases (MMPs) collagenase, gelatinase A (72 kDa gelatinase), stromelysin, and their specific inhibitor TIMP-1 (tissue inhibitor of metalloproteinases), were immunolocalized using specific polyclonal antisera in gingival tissues from 21 patients with chronic inflammatory periodontal disease. Monoclonal antibodies against macrophages (Leu-M5), B cells (Leu-14), helper T cells (OKT4), suppressor T cells (OKT8) and the HLA-DR epitope were also used to identify leukocyte subsets. MMPs were observed in connective tissues at sites that histologically showed signs of remodelling. The number and distribution of positive cells varied widely, however, not only between individual biopsy specimens, but also within the same specimen. The same was true for the composition and distribution of the inflammatory cell infiltrate. Moreover, although there was a positive correlation between the number of MMP-producing cells and the severity of inflammation in some specimens, for others with comparable leukocyte subset scoring the number was reduced and sometimes absent altogether. Cells secreting MMPs were fibroblasts, macrophages and epithelial cells. It was not possible to determine unequivocally whether a MMP-positive cell within the connective tissue was a fibroblast or a macrophage, since the antisera recognise both fibroblast and macrophage MMPs and the different fixation requirements for MMPs (4% paraformaldehyde) and Leu-M5 (acetone) precluded co-localization on the same section. TIMP-1 was immunolocalized within connective tissue cells at sites of tissue remodelling. Our results support the hypothesis that tissue-derived MMPs may be involved in tissue remodelling in periodontal disease and conclusively demonstrate that epithelial cells may be involved as well as connective tissue cells.
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人角质形成细胞在培养物中组成型产生前胶原酶和金属蛋白酶组织抑制剂。
DOI：
10.1111/1523-1747.ep12276678
发表时间：
1989
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Petersen,MJ;Woodley,DT;Stricklin,GP;O'Keefe,EJ
通讯作者：
O'Keefe,EJ
疑似人类牙周病原体激活角质形成细胞介导的胶原蛋白（I 型）分解。
影响因子：
3.5
作者：
Birkedal-Hansen,H;Wells,BR;Lin,HY;Caufield,PW;Taylor,RE
通讯作者：
Taylor,RE
成人角质形成细胞在非活性真皮胶原蛋白上迁移，产生胶原蛋白分解酶，可降解 I 型和 IV 型胶原蛋白。
DOI：
10.1111/1523-1747.ep12285689
发表时间：
1986
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Woodley,DT;Kalebec,T;Banes,AJ;Link,W;Prunieras,M;Liotta,L
通讯作者：
Liotta,L
DOI：
10.1172/jci116073
发表时间：
1992-11
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
U. K. Saarialho-Kere;E. S. Chang;H. Welgus;William C. Parks
通讯作者：
U. K. Saarialho-Kere;E. S. Chang;H. Welgus;William C. Parks