Characterization and Activity Analyses of the FLOWERING LOCUS T Promoter in Gossypium Hirsutum
Characterization and Activity Analyses of the FLOWERING LOCUS T Promoter in Gossypium Hirsutum
复制标题
陆地棉 FLOWERING LOCUS T 启动子的特性及活性分析
DOI：
10.3390/ijms20194769
复制
发表时间：
2019-09
影响因子：
5.6
通讯作者：
Xianzhong Huang
中科院分区：
文献类型：
--
作者：
Na Sang;Darun Cai;Chao Li;Yuqiang Sun;Xianzhong Huang
开花转换是陆地棉（Gossypium hirsutum L.）重要的发育过程，开花时间与FLOW LOCUS T（FT）的表达时间密切相关。然而，棉花FT启动子中各种顺式调控元件的协调机制尚未确定。在这项研究中，从棉花中鉴定了FT的5.9 kb启动子。生物信息学分析表明，该启动子存在多个插入-缺失位点。不同的报告基因的表达水平，和诱导在GhFT启动子中的顺序缺失，表明1.8 kb的GhFT启动子是强于4.2-，4.8-和5.9-kb的启动子片段。CONSTANS（CO）和核因子Y转录因子的结合位点位于FT转录起始位点上游的1.0 kb序列内。在近端启动子区域（-1.1至-1.4 kb）鉴定出大量重复片段。对G.陆地棉、亚洲棉和雷蒙棉FT启动子的研究表明，1.0-kb片段显著拯救了拟南芥FT功能丧失突变体ft-10的晚花表型，而1.8-kb启动子仅轻微拯救了晚花表型。此外，棉花FT启动子中保守的CORE基序是一个非典型的TGTG（N2-3）ATG，但TGTG和ATG之间的任意碱基数目是不确定的。因此，近端FT启动子区可能在影响棉花开花过程中FT启动子活性水平方面起重要作用。
Flowering transition is a crucial development process in cotton (Gossypium hirsutum L.), and the flowering time is closely correlated with the timing of FLOWERING LOCUS T (FT) expression. However, the mechanism underlying the coordination of various cis-regulatory elements in the FT promoter of cotton has not been determined. In this study, a 5.9-kb promoter of FT was identified from cotton. A bioinformatics analysis showed that multiple insertion–deletion sites existed in the 5.9-kb promoter. Different expression levels of a reporter gene, and the induction by sequential deletions in GhFT promoter, demonstrated that 1.8-kb of the GhFT promoter was stronger than 4.2-, 4.8-, and 5.9-kb promoter fragments. The binding sites of the CONSTANS (CO) and NUCLEAR FACTOR Y transcription factors were located within the 1.0-kb sequence upstream of the FT transcription start site. A large number of repeat segments were identified in proximal promoter regions (−1.1 to −1.4 kb). A complementation analysis of deletion constructs between 1.0 and 1.8 kb of G. hirsutum, Gossypium arboretum, and Gossypium raimondii FT promoters revealed that the 1.0-kb fragment significantly rescued the late-flowering phenotype of the Arabidopsis FT loss-of-function mutant ft-10, whereas the 1.8-kb promoter only slightly rescued the late-flowering phenotype. Furthermore, the conserved CORE motif in the cotton FT promoter is an atypical TGTG(N2-3)ATG, but the number of arbitrary bases between TGTG and ATG is uncertain. Thus, the proximal FT promoter region might play an important role affecting the activity levels of FT promoters in cotton flowering.
登录
查看更多内容
影响因子：
3.7
作者：
Zhang X;Wang C;Pang C;Wei H;Wang H;Song M;Fan S;Yu S
通讯作者：
Yu S
多种 bHLH 蛋白形成异二聚体，介导拟南芥开花时间的 CRY2 依赖性调节。
影响因子：
4.5
作者：
Liu Y;Li X;Li K;Liu H;Lin C
通讯作者：
Lin C
陆地棉 (Gossypium hirsutum L.) 类 CONSTANS (COL) 基因家族的鉴定和表征
影响因子：
3.7
作者：
Cai D;Liu H;Sang N;Huang X
通讯作者：
Huang X
影响因子：
7.4
作者：
Hayama, R;Coupland, G
通讯作者：
Coupland, G
影响因子：
11.8
作者：
Li, Dan;Liu, Chang;Yu, Hao
通讯作者：
Yu, Hao