Proteomic analysis of an unculturable bacterial endosymbiont (Blochmannia) reveals high abundance of chaperonins and biosynthetic enzymes.
Proteomic analysis of an unculturable bacterial endosymbiont (Blochmannia) reveals high abundance of chaperonins and biosynthetic enzymes.
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DOI：
10.1021/pr3007842
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发表时间：
2013-02-01
影响因子：
4.4
通讯作者：
Wernegreen JJ
中科院分区：
文献类型：
--
作者：
Fan Y;Thompson JW;Dubois LG;Moseley MA;Wernegreen JJ
许多昆虫群体与生活在特化宿主细胞内的细菌内共生体共同进化。作为一个突出的例子，Camponotini部落的蚂蚁依赖于Blochmannia，这是一种细胞内的细菌互利主义者，可以合成氨基酸并为宿主提供氮。我们进行了鸟枪，无标记，LC/MS/MS定量蛋白质组学分析，以探讨与Camponotus chromiodes相关的Blochmannia蛋白质组。我们确定了超过330个Blochmannia蛋白，或预测的蛋白质组的54%的覆盖率，以及244 Camponotus蛋白。使用前3个“最佳飞行”肽沿着的平均强度，并加标已知浓度的替代标准品，我们估计了这些蛋白质的浓度（fmol/μg），具有可靠的鉴别。Blochmannia蛋白质丰度的估计动态范围跨越三个数量级，涵盖了不同的功能类别，特别是高代表性的代谢，信息传递和分子伴侣。GroEL是最丰富的蛋白质，占Blochmannia蛋白质丰度的6%。必需氨基酸、脂肪酸和核苷酸的生物合成以及硫酸盐同化在蛋白质组中的覆盖率不成比例地高，进一步支持了共生的营养作用。这第一个定量蛋白质组学分析的蚂蚁内共生体说明了一个有前途的方法来研究亲密共生的功能基础。
Many insect groups have coevolved with bacterial endosymbionts that live within specialized host cells. As a salient example, ants in the tribe Camponotini rely on Blochmannia, an intracellular bacterial mutualist that synthesizes amino acids and recycles nitrogen for the host. We performed a shotgun, label-free, LC/MS/MS quantitative proteomic analysis to investigate the proteome of Blochmannia associated with Camponotus chromaiodes. We identified more than 330 Blochmannia proteins, or 54% coverage of the predicted proteome, as well as 244 Camponotus proteins. Using the average intensity of the top 3 “best flier” peptides along with spiking of a surrogate standard at a known concentration, we estimated the concentration (fmol/μg) of those proteins with confident identification. The estimated dynamic range of Blochmannia protein abundance spanned three orders of magnitude and covered diverse functional categories, with particularly high representation of metabolism, information transfer, and chaperones. GroEL, the most abundant protein, totaled 6% of Blochmannia protein abundance. Biosynthesis of essential amino acids, fatty acids, and nucleotides, and sulfate assimilation had disproportionately high coverage in the proteome, further supporting a nutritional role of the symbiosis. This first quantitative proteomic analysis of an ant endosymbiont illustrates a promising approach to study the functional basis of intimate symbioses.
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DOI：
10.1006/bbrc.1997.7552
发表时间：
1997-10-29
影响因子：
3.1
作者：
Charles, H;Heddi, A;Nardon, P
通讯作者：
Nardon, P
影响因子：
4.5
作者：
Ghazalpour A;Bennett B;Petyuk VA;Orozco L;Hagopian R;Mungrue IN;Farber CR;Sinsheimer J;Kang HM;Furlotte N;Park CC;Wen PZ;Brewer H;Weitz K;Camp DG 2nd;Pan C;Yordanova R;Neuhaus I;Tilford C;Siemers N;Gargalovic P;Eskin E;Kirchgessner T;Smith DJ;Smith RD;Lusis AJ
通讯作者：
Lusis AJ
影响因子：
7
作者：
Griffin, TJ;Gygi, SP;Aebersold, R
通讯作者：
Aebersold, R
影响因子：
30.8
作者：
Akman, L;Yamashita, A;Aksoy, S
通讯作者：
Aksoy, S
影响因子：
2.6
作者：
Aksoy, S.
通讯作者：
Aksoy, S.