SNP discovery and linkage map construction in cultivated tomato.
SNP discovery and linkage map construction in cultivated tomato.
复制标题
DOI：
10.1093/dnares/dsq024
复制
发表时间：
2010-12
期刊：
影响因子：
--
通讯作者：
Tabata S
中科院分区：
文献类型：
--
作者：
Shirasawa K;Isobe S;Hirakawa H;Asamizu E;Fukuoka H;Just D;Rothan C;Sasamoto S;Fujishiro T;Kishida Y;Kohara M;Tsuruoka H;Wada T;Nakamura Y;Sato S;Tabata S
栽培番茄种内遗传连锁图谱的研究很少，主要是因为番茄种内遗传多样性远低于番茄种间遗传多样性。单核苷酸多态性（SNPs），基因组变异的最丰富的来源，是最有前途的多态性的来源，为密切相关的种内品系的连锁图谱的构建。在这项研究中，我们开发了基于表达序列标签的SNP标记，用于构建番茄种内连锁图谱。在通过计算机模拟分析检测的5607个SNP位点中，选择1536个位点用于来源于“Micro-Tom”与“Ailsa克雷格”或“M82”之间杂交的两个作图群体的高通量基因分型。共将1137个标记（包括1338个成功分型的SNPs中的793个）和344个简单重复序列和内含子多态性标记（沿着）定位到两张连锁图上，图谱覆盖范围分别为1467.8和1422.7 cM。然后对栽培番茄品系筛选开发的SNP标记，以估计这些SNP对其他育种材料的可转移性。分子标记和连锁图谱的建立是基因组学和遗传学研究的一个里程碑，也是栽培番茄分子育种的第一步。有关这些番茄品系的DNA标记、连锁图谱和SNP基因型的信息可在。
Few intraspecific genetic linkage maps have been reported for cultivated tomato, mainly because genetic diversity within Solanum lycopersicum is much less than that between tomato species. Single nucleotide polymorphisms (SNPs), the most abundant source of genomic variation, are the most promising source of polymorphisms for the construction of linkage maps for closely related intraspecific lines. In this study, we developed SNP markers based on expressed sequence tags for the construction of intraspecific linkage maps in tomato. Out of the 5607 SNP positions detected through in silico analysis, 1536 were selected for high-throughput genotyping of two mapping populations derived from crosses between ‘Micro-Tom’ and either ‘Ailsa Craig’ or ‘M82’. A total of 1137 markers, including 793 out of the 1338 successfully genotyped SNPs, along with 344 simple sequence repeat and intronic polymorphism markers, were mapped onto two linkage maps, which covered 1467.8 and 1422.7 cM, respectively. The SNP markers developed were then screened against cultivated tomato lines in order to estimate the transferability of these SNPs to other breeding materials. The molecular markers and linkage maps represent a milestone in the genomics and genetics, and are the first step toward molecular breeding of cultivated tomato. Information on the DNA markers, linkage maps, and SNP genotypes for these tomato lines is available at .
登录
查看更多内容
影响因子：
30.8
作者：
Cong, Bin;Barrero, Luz S.;Tanksley, Steven D.
通讯作者：
Tanksley, Steven D.
影响因子：
1.6
作者：
Matsukura, Chiaki;Yamaguchi, Isao;Nishimura, Shigeo
通讯作者：
Nishimura, Shigeo
影响因子：
5.4
作者：
MILLER, JC;TANKSLEY, SD
通讯作者：
TANKSLEY, SD
影响因子：
3.1
作者：
Ohyama, Akio;Asamizu, Erika;Fukuoka, Hiroyuki
通讯作者：
Fukuoka, Hiroyuki
影响因子：
7.2
作者：
Meissner, R;Jacobson, Y;Levy, A
通讯作者：
Levy, A