A Full-Length Reference Floral Transcriptome of Boehmeria tricuspis Provides Insights into Apomeiosis and Polyploidy
A Full-Length Reference Floral Transcriptome of Boehmeria tricuspis Provides Insights into Apomeiosis and Polyploidy
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苎麻的全长参考花转录组提供了对无性减数分裂和多倍体的见解
DOI：
10.1155/2019/4025747
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发表时间：
2019-12
期刊：
影响因子：
--
通讯作者：
Jianguang Su
中科院分区：
文献类型：
--
作者：
Qing Tang;Ying Xu;Canhui Deng;Chaohua Cheng;Zhigang Dai;Zemao Yang;Chan Liu;Jianguang Su
三裂麻Boehmeria tricuspis(Hance)Makino是一种原产于东亚的耐寒草本或灌木状多年生草本植物，具有不同的倍性水平和繁殖方式(双孢子到有性)。虽然已经描述了几个与无融合生殖相关的基因，但无融合生殖的遗传控制和分子机制仍然知之甚少。此外，多倍体和无融合生殖之间的相关性的基础还没有弄清楚。我们利用长阅读测序技术获得了三尖杉的全长参考花转录组。基于所建立的数据库，比较了不同倍性水平和繁殖方式细胞型的雌花的基因表达。共鉴定出1387个与无融合生殖有关的基因，217个与倍性有关的基因，9个与无融合生殖和倍性有关的基因。对这组转录本的基因本体论分析表明，生殖基因，特别是那些与“细胞分化”和“细胞周期过程”相关的基因，是调节无融合生殖的重要因素。此外，我们的结果表明，胁迫反应基因的不同表达可能在无融合生殖转换的准备中发挥重要作用。此外，我们的观察表明，无融合生殖的表达可能不依赖于多倍体，而是依赖于性途径的解除。
Boehmeria tricuspis (Hance) Makino constitutes a hardy herbaceous or shrubby perennial native to East Asia that includes different ploidy levels and reproductive modes (diplosporous to sexual). Although several apomeiosis-associated genes have been described, the genetic control and molecular mechanisms underlying apomeiosis remain poorly understood. Moreover, the basis of the correlation between polyploidy and apomixis has not yet been clarified. We utilized long-read sequencing to produce a full-length reference floral transcriptome of B. tricuspis. Based on the generated database, gene expression of the female flowers of different ploidy levels and reproductive mode cytotypes was compared. Overall, 1,387 genes related to apomeiosis, 217 genes related to ploidy, and 9 genes associated with both apomixis and ploidy were identified. Gene Ontology analyses of this set of transcripts indicated reproductive genes, especially those related to "cell differentiation" and "cell cycle process," as significant factors regulating apomeiosis. Furthermore, our results suggested that different expressions of stress response genes might be important in the preparation for apomeiosis transition. In addition, our observations indicated that the expression of apomeiosis may not depend on polyploidy but rather on deregulation of the sexual pathway in B. tricuspis.
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影响因子：
5.2
作者：
L. Martelotto;J. P. Ortiz;J. Stein;F. Espinoza;C. Quarin;S. Pessino
通讯作者：
L. Martelotto;J. P. Ortiz;J. Stein;F. Espinoza;C. Quarin;S. Pessino
影响因子：
3.4
作者：
Hoerandl, Elvira;Hadacek, Franz
通讯作者：
Hadacek, Franz
影响因子：
5.6
作者：
Klatt S;Hadacek F;Hodač L;Brinkmann G;Eilerts M;Hojsgaard D;Hörandl E
通讯作者：
Hörandl E
影响因子：
3.3
作者：
I. Golubovskaya;Z. K. Grebennikova;N. Avalkina;W. Sheridan
通讯作者：
I. Golubovskaya;Z. K. Grebennikova;N. Avalkina;W. Sheridan
影响因子：
5.1
作者：
Albertini, E;Marconi, G;Falcinelli, M
通讯作者：
Falcinelli, M