Use of electric cell-substrate impedance sensing to assess in vitro cytotoxicity.
Use of electric cell-substrate impedance sensing to assess in vitro cytotoxicity.
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DOI：
10.1016/j.bios.2009.01.015
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发表时间：
2009-04-15
影响因子：
12.6
通讯作者：
Lo CM
中科院分区：
文献类型：
--
作者：
Opp D;Wafula B;Lim J;Huang E;Lo JC;Lo CM
基于电化学阻抗谱（EIS）的细胞毒性体外评估需要更多的定量方法来分析细胞形态和运动的改变，从而分析对人类健康的潜在风险。在这里，我们应用电细胞基质阻抗传感（ECIS）来评估暴露于细胞松弛素B的人脐静脉内皮细胞的剂量依赖性反应。为了检测细胞形态的细微变化，用理论细胞电极模型测量和分析了细胞单层的频率依赖性阻抗数据。为了检测响应于细胞松弛素B激发的细胞微动的交替，监测细胞覆盖的电极的时间序列阻抗波动，并计算功率谱、方差和增量的方差的值以验证差异。虽然通常从细胞单层的总电阻观察到剂量依赖性关系，但频率依赖性阻抗和阻抗波动分析区分了低至0.1 μM的细胞松弛素B水平。我们的研究结果表明，细胞松弛素B引起细胞间连接电阻的降低，膜电容的增加，和微动减少。
In vitro assessment of cytotoxicity based on electrochemical impedance spectroscopy (EIS) needs more quantitative methods to analyze the alteration of cell morphology and motility, and hence the potential risk to human health. Here, we applied electric cell-substrate impedance sensing (ECIS) to evaluate dose-dependent responses of human umbilical vein endothelial cells exposed to cytochalasin B. To detect subtle changes in cell morphology, the frequency-dependent impedance data of the cell monolayer were measured and analyzed with a theoretical cell-electrode model. To detect the alternation of cell micromotion in response to cytochalasin B challenge, time-series impedance fluctuations of cell-covered electrodes were monitored and the values of power spectrum, variance, and variance of the increments were calculated to verify the difference. While a dose-dependent relationship was generally observed from the overall resistance of the cell monolayer, the analysis of frequency-dependent impedance and impedance fluctuations distinguished cytochalasin B levels as low as 0.1 μM. Our results show that cytochalasin B causes a decrease of junctional resistance between cells, an increase of membrane capacitance, and the reduction in micromotion.
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影响因子：
7.8
作者：
Brown, S S;Spudich, J A
通讯作者：
Spudich, J A
影响因子：
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作者：
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DOI：
10.1007/s002160000628
发表时间：
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期刊：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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