Establishment of basal cell carcinoma animal model in Chinese tree shrew (Tupaia belangeri chinensis).
Establishment of basal cell carcinoma animal model in Chinese tree shrew (Tupaia belangeri chinensis).
复制标题
中国树鼩基底细胞癌动物模型的建立
DOI：
10.24272/j.issn.2095-8137.2017.045
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发表时间：
2017-07-18
影响因子：
4.9
通讯作者：
Chen YB
中科院分区：
文献类型：
--
作者：
Jiang LP;Shen QS;Yang CP;Chen YB
基底细胞癌（BCC）是世界上最常见的皮肤癌，发病率持续增加。紫外线辐射是主要的环境危险因素，并且在大多数bcc中发现了Hedgehog （Hh）信号通路的失调。局部晚期和转移性BBCs的治疗仍然是一个挑战，需要比广泛使用的啮齿动物更好的动物模型来进行药物开发和测试。中国树鼩（Tupaia belangeri chinensis）与灵长类动物有密切的亲缘关系，在表征人类疾病方面比啮齿类动物具有许多生理生化优势。在这里，我们通过用慢病毒SmoA1感染中国树鼩尾部皮肤成功建立了BCC模型，SmoA1是一种活性形式的Smoothened (Smo)，用于组成性激活Hh信号通路。免疫组织化学分析证实病理特征。有趣的是，联合使用针对中国树鼩p53的慢病毒SmoA1和shRNA，大大促进了BCC的进展。这项工作为进一步的BCC研究和未来的药物发现提供了有用的动物模型。
Basal cell carcinoma (BCC) is the most common skin cancer worldwide, with incidence rates continuing to increase. Ultraviolet radiation is the major environmental risk factor and dysregulation of the Hedgehog (Hh) signaling pathway has been identified in most BCCs. The treatment of locally advanced and metastatic BBCs is still a challenge and requires a better animal model than the widely used rodents for drug development and testing. Chinese tree shrews (Tupaia belangeri chinensis) are closely related to primates, bearing many physiological and biochemical advantages over rodents for characterizing human diseases. Here, we successfully established a Chinese tree shrew BCC model by infecting tail skins with lentiviral SmoA1, an active form of Smoothened (Smo) used to constitutively activate the Hh signaling pathway. The pathological characteristics were verified by immunohistochemical analysis. Interestingly, BCC progress was greatly enhanced by the combined usage of lentiviral SmoA1 and shRNA targeting Chinese tree shrew p53. This work provides a useful animal model for further BCC studies and future drug discoveries.
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