What properties characterize the hub proteins of the protein-protein interaction network of Saccharomyces cerevisiae?
What properties characterize the hub proteins of the protein-protein interaction network of Saccharomyces cerevisiae?
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DOI：
10.1186/gb-2006-7-6-r45
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发表时间：
2006
期刊：
影响因子：
12.3
通讯作者：
Elofsson A
中科院分区：
文献类型：
--
作者：
Ekman D;Light S;Björklund AK;Elofsson A
对酿酒酵母蛋白相互作用网络中的枢纽蛋白（具有许多相互作用物的蛋白）和非枢纽蛋白的分析表明，枢纽蛋白富含多个重复结构域。大多数蛋白质只与少数其他蛋白质相互作用，而少数蛋白质（枢纽）有许多相互作用的伙伴。枢纽蛋白和非枢纽蛋白在几个方面有所不同；然而，对于集线器的特性以及将它们与低连通性的蛋白质区分开来的理解并不完整。因此，我们研究了酿酒酵母蛋白质-蛋白质相互作用网络中的中心与非中心、静态中心（聚会中心）与动态中心（约会中心）的区别。枢纽蛋白的许多相互作用只能部分地通过与类似蛋白质或结构域的结合来解释。很明显，与结合相关的结构域重复序列在枢纽中丰富。此外，在枢纽之间存在多结构域蛋白和长蛋白的过度表示。此外，聚会中心和约会中心之间也有明显的区别。与日期中心相比，聚会中心包含长无序区域的数量较少，这表明这些区域对于灵活绑定很重要，但对于静态交互则不那么重要。此外，聚会中心在很大程度上相互作用，支持聚会中心作为高度集群功能模块核心的想法。此外，枢纽蛋白，尤其是聚会枢纽，往往更古老。最后，最近类似的政党中心的代表性不足。我们的研究结果表明，中心蛋白中富含多个重复结构域，此外，在枣中心中常见的长无序区域对灵活结合尤为重要。
An analysis of hubs (proteins with many interactors) and non-hubs in the S. cerevisiae protein interaction network shows that hub proteins are enriched with multiple and repeated domains. Most proteins interact with only a few other proteins while a small number of proteins (hubs) have many interaction partners. Hub proteins and non-hub proteins differ in several respects; however, understanding is not complete about what properties characterize the hubs and set them apart from proteins of low connectivity. Therefore, we have investigated what differentiates hubs from non-hubs and static hubs (party hubs) from dynamic hubs (date hubs) in the protein-protein interaction network of Saccharomyces cerevisiae. The many interactions of hub proteins can only partly be explained by bindings to similar proteins or domains. It is evident that domain repeats, which are associated with binding, are enriched in hubs. Moreover, there is an over representation of multi-domain proteins and long proteins among the hubs. In addition, there are clear differences between party hubs and date hubs. Fewer of the party hubs contain long disordered regions compared to date hubs, indicating that these regions are important for flexible binding but less so for static interactions. Furthermore, party hubs interact to a large extent with each other, supporting the idea of party hubs as the cores of highly clustered functional modules. In addition, hub proteins, and in particular party hubs, are more often ancient. Finally, the more recent paralogs of party hubs are underrepresented. Our results indicate that multiple and repeated domains are enriched in hub proteins and, further, that long disordered regions, which are common in date hubs, are particularly important for flexible binding.
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DOI：
10.1073/pnas.231384098
发表时间：
2001-10-23
影响因子：
11.1
作者：
Malhotra, R;Holman, M;Ito, T
通讯作者：
Ito, T
影响因子：
64.8
作者：
Gavin, AC;Bösche, M;Superti-Furga, G
通讯作者：
Superti-Furga, G
影响因子：
64.8
作者：
Jeong, H;Mason, SP;Oltvai, ZN
通讯作者：
Oltvai, ZN
影响因子：
64.8
作者：
Albert, R;Jeong, H;Barabási, AL
通讯作者：
Barabási, AL
影响因子：
10.7
作者：
Kunin, V;Pereira-Leal, JB;Ouzounis, CA
通讯作者：
Ouzounis, CA