Detection of T. urartu Introgressions in Wheat and Development of a Panel of Interspecific Introgression Lines.
Detection of T. urartu Introgressions in Wheat and Development of a Panel of Interspecific Introgression Lines.
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DOI：
10.3389/fpls.2018.01565
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发表时间：
2018
影响因子：
5.6
通讯作者：
King J
中科院分区：
文献类型：
--
作者：
Grewal S;Hubbart-Edwards S;Yang C;Scholefield D;Ashling S;Burridge A;Wilkinson PA;King IP;King J
Tritcumurartu（2n = 2x = 14，AuAu）是小麦A基因组的供体，由于其高光合速率和抗病性，是小麦遗传改良新变异的重要来源。通过促进全基因组渐渗的产生，导致各种不同的小麦-T。urartu易位系、T. urartu在小麦改良中具有实用价值。以前的研究已经产生了这样的渐渗系，一直无法成功地使用细胞学方法来检测T。在这些线路上的Urartu。因此，许多人已经使用了各种分子标记，但由于这些标记的低密度覆盖率和技术的耗时性质使得它们不适合大规模育种计划，因此成功有限。在这项研究中，我们报告了单核苷酸多态性（SNP）标记资源的产生，存在于高通量SNP基因分型阵列上，可以检测T。urartu在六倍体小麦背景中的应用使其成为小麦预育种程序中潜在的有价值的工具。全基因组渐渗方法已经导致来自T. urartu导入小麦，然后使用这些SNP标记检测和表征。对这些小麦-T. urartu重组株系的构建，导致了T. urartu包含368个SNP标记，分布在T.乌拉图。比较分析了T. urartu和六倍体小麦基因组物理图谱的比较表明，两个物种之间的同线性在宏观水平上是高度保守的，并证实了T. urartu也存在于小麦的A基因组中。一组17个小麦-T。urartu重组系，由覆盖T. urartu也被选择用于自花受精，以提供用于未来性状分析的种质资源。这一宝贵的高密度分子标记资源和一组稳定的种间渐渗系将大大提高小麦野生近缘基因渐渗改良的效率。
Tritcum urartu (2n = 2x = 14, AuAu), the A genome donor of wheat, is an important source for new genetic variation for wheat improvement due to its high photosynthetic rate and disease resistance. By facilitating the generation of genome-wide introgressions leading to a variety of different wheat–T. urartu translocation lines, T. urartu can be practically utilized in wheat improvement. Previous studies that have generated such introgression lines have been unable to successfully use cytological methods to detect the presence of T. urartu in these lines. Many have, thus, used a variety of molecular markers with limited success due to the low-density coverage of these markers and time-consuming nature of the techniques rendering them unsuitable for large-scale breeding programs. In this study, we report the generation of a resource of single nucleotide polymorphic (SNP) markers, present on a high-throughput SNP genotyping array, that can detect the presence of T. urartu in a hexaploid wheat background making it a potentially valuable tool in wheat pre-breeding programs. A whole genome introgression approach has resulted in the transfer of different chromosome segments from T. urartu into wheat which have then been detected and characterized using these SNP markers. The molecular analysis of these wheat-T. urartu recombinant lines has resulted in the generation of a genetic map of T. urartu containing 368 SNP markers, spread across all seven chromosomes of T. urartu. Comparative analysis of the genetic map of T. urartu and the physical map of the hexaploid wheat genome showed that synteny between the two species is highly conserved at the macro-level and confirmed the presence of the 4/5 translocation in T. urartu also present in the A genome of wheat. A panel of 17 wheat-T. urartu recombinant lines, which consisted of introgressed segments that covered the whole genome of T. urartu, were also selected for self-fertilization to provide a germplasm resource for future trait analysis. This valuable resource of high-density molecular markers specifically designed for detecting wild relative chromosomes and a panel of stable interspecific introgression lines will greatly enhance the efficiency of wheat improvement through wild relative introgressions.
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影响因子：
12.3
作者：
Alaux M;Rogers J;Letellier T;Flores R;Alfama F;Pommier C;Mohellibi N;Durand S;Kimmel E;Michotey C;Guerche C;Loaec M;Lainé M;Steinbach D;Choulet F;Rimbert H;Leroy P;Guilhot N;Salse J;Feuillet C;International Wheat Genome Sequencing Consortium;Paux E;Eversole K;Adam-Blondon AF;Quesneville H
通讯作者：
Quesneville H
影响因子：
1.6
作者：
Alvarez, J. B.;Caballero, L.;Martin, A.
通讯作者：
Martin, A.
影响因子：
1.5
作者：
CHAPMAN, V;MILLER, TE;RILEY, R
通讯作者：
RILEY, R
影响因子：
2.6
作者：
Fricano, Agostino;Brandolini, Andrea;Salamini, Francesco
通讯作者：
Salamini, Francesco
通过全基因组渗入小麦的脑膜膜染色体表征。
DOI：
10.1007/s00122-017-3009-y
发表时间：
2018-03
期刊：
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
影响因子：
--
作者：
Grewal S;Yang C;Edwards SH;Scholefield D;Ashling S;Burridge AJ;King IP;King J
通讯作者：
King J