Caloric restriction delays aging-induced cellular phenotypes in rhesus monkey skeletal muscle.
Caloric restriction delays aging-induced cellular phenotypes in rhesus monkey skeletal muscle.
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DOI：
10.1016/j.exger.2010.09.011
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发表时间：
2011-01
影响因子：
3.9
通讯作者：
Weindruch, Richard
中科院分区：
文献类型：
--
作者：
McKiernan, Susan H.;Colman, Ricki J.;Lopez, Marisol;Beasley, T. Mark;Aiken, Judd M.;Anderson, Rozalyn M.;Weindruch, Richard
肌肉减少症是骨骼肌质量和功能的年龄相关性损失，其特征在于肌肉质量和纤维横截面积减少、肌纤维类型改变和线粒体功能变化。在恒河猴中，没有营养不良的热量限制（CR）改善了生存率，并延迟了年龄相关疾病和障碍（包括肌肉减少症）的发作。我们提出了一个纵向研究的影响CR早期肌肉减少症的上腿从16岁到22岁的猴子。使用双能X射线吸收法，我们表明，CR延迟最大肌肉质量的发展，与对照组动物不同，在早期阶段肌肉减少症的CR动物的大腿肌肉质量得到保留。股外侧肌活检组织化学分析显示，CR反对II型肌纤维和纤维横截面积的比例与年龄相关的变化。相反，CR对线粒体电子传递系统酶异常（ETSab）的肌纤维数量没有显着影响。ETSab纤维的激光捕获显微切割和随后的线粒体DNA的PCR分析揭示了具有异常线粒体酶活性的纤维中的大缺失突变。CR并不能阻止肌纤维中的随机线粒体缺失突变，但CR可能有助于维持受影响的纤维。
Sarcopenia is the age-related loss of skeletal muscle mass and function and is characterized by a reduction in muscle mass and fiber cross-sectional area, alterations in muscle fiber type and mitochondrial functional changes. In rhesus monkeys, calorie restriction (CR) without malnutrition improves survival and delays the onset of age-associated diseases and disorders including sarcopenia. We present a longitudinal study on the impact of CR on early stage sarcopenia in the upper leg of monkeys from ~16 years to ~22 years of age. Using dual-energy X-ray absorptiometry we show that CR delayed the development of maximum muscle mass and, unlike Control animals, muscle mass of the upper leg was preserved in CR animals during early phase sarcopenia. Histochemical analyses of vastus lateralis muscle biopsies revealed that CR opposed age-related changes in the proportion of Type II muscle fibers and fiber cross-sectional area. In contrast the number of muscle fibers with mitochondrial electron transport system enzyme abnormalities (ETSab) was not significantly affected by CR. Laser capture microdissection of ETSab fibers and subsequent PCR analysis of the mitochondrial DNA revealed large deletion mutations in fibers with abnormal mitochondrial enzyme activities. CR did not prevent stochastic mitochondrial deletion mutations in muscle fibers but CR may have contributed to the maintenance of affected fibers.
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DOI：
10.1126/science.1173635
发表时间：
2009-07-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colman RJ;Anderson RM;Johnson SC;Kastman EK;Kosmatka KJ;Beasley TM;Allison DB;Cruzen C;Simmons HA;Kemnitz JW;Weindruch R
通讯作者：
Weindruch R
DOI：
10.1016/j.tem.2009.11.005
发表时间：
2010-03
期刊：
Trends in endocrinology and metabolism: TEM
影响因子：
--
作者：
Anderson RM;Weindruch R
通讯作者：
Weindruch R
DOI：
10.1139/h02-023
发表时间：
2002-08-01
期刊：
CANADIAN JOURNAL OF APPLIED PHYSIOLOGY-REVUE CANADIENNE DE PHYSIOLOGIE APPLIQUEE
影响因子：
--
作者：
Pette, D
通讯作者：
Pette, D
影响因子：
2.5
作者：
Degens, H;Alway, SE
通讯作者：
Alway, SE
DOI：
10.1093/geronj/48.1.b17
发表时间：
1993-01-01
期刊：
JOURNALS OF GERONTOLOGY
影响因子：
--
作者：
KEMNITZ, JW;WEINDRUCH, R;ERSHLER, WB
通讯作者：
ERSHLER, WB