Reporter Virus Neutralization Test Evaluation for Dengue and Zika Virus Diagnosis in Flavivirus Endemic Area.
Reporter Virus Neutralization Test Evaluation for Dengue and Zika Virus Diagnosis in Flavivirus Endemic Area.
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DOI：
10.3390/pathogens10070840
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发表时间：
2021-07-03
期刊：
影响因子：
--
通讯作者：
Medeiros DBA
中科院分区：
文献类型：
--
作者：
Nunes JGC;Nunes BTD;Shan C;Moraes AF;Silva TR;de Mendonça MHR;das Chagas LL;Silva FAE;Azevedo RSS;da Silva EVP;Martins LC;Chiang JO;Casseb LMN;Henriques DF;Vasconcelos PFC;Burbano RMR;Shi PY;Medeiros DBA
报道病毒中和试验（RVNT）已被用作DENV和ZIKV两者的更费力和耗时的常规PRNT测定的替代方法。然而，很少有研究调查这些技术将如何在流行地区与多种黄病毒的循环。在此，我们评估了ZIKV和DENV Rluc RVNT和ZIKV mCh RVNT测定与常规PRNT测定相比针对在巴西ZIKV爆发之前和期间收集的患者血清的性能。基于（1）根据疾病时间的急性和恢复期样品，和（2）实验室诊断结果（DENV和ZIKV RT-PCR和IgM捕获ELISA），将这些样品分类成组。结果表明，DENV Rluc检测的敏感性和特异性分别为100%和78.3%，准确性为93.3%，与传统的PRNT性能相似。另一方面，与PRNT相比，ZIKV RVNT 90显示出更好的ZIKV抗体检测性能（高约9倍），具有88%的临床灵敏度。特异性值平均为76.8%。然而，即使有这些结果，由于黄病毒交叉反应，单独的ZIKV RVNT 90也不能达到人样品中继发感染的最终诊断结论。因此，在黄病毒鉴别诊断具有挑战性的地区，我们建议建立包括该地区流行的其他黄病毒的RVNT面板，与其他血清学技术（如IgM ELISA）和血清转化调查相关，以帮助使用血清学确定准确的诊断结论。
Reporter virus neutralization test (RVNT) has been used as an alternative to the more laborious and time-demanding conventional PRNT assay for both DENV and ZIKV. However, few studies have investigated how these techniques would perform in epidemic areas with the circulation of multiple flavivirus. Here, we evaluate the performance of ZIKV and DENV Rluc RVNT and ZIKV mCh RVNT assays in comparison to the conventional PRNT assay against patient sera collected before and during ZIKV outbreak in Brazil. These samples were categorized into groups based on (1) acute and convalescent samples according to the time of disease, and (2) laboratorial diagnostic results (DENV and ZIKV RT-PCR and IgM-capture ELISA). Our results showed that DENV Rluc assay presented 100% and 78.3% sensitivity and specificity, respectively, with 93.3% accuracy, a similar performance to the traditional PRNT. ZIKV RVNT90, on the other hand, showed much better ZIKV antibody detection performance (around nine-fold higher) when compared to PRNT, with 88% clinical sensitivity. Specificity values were on average 76.8%. Even with these results, however, ZIKV RVNT90 alone was not able to reach a final diagnostic conclusion for secondary infection in human samples due to flavivirus cross reaction. As such, in regions where the flavivirus differential diagnosis represents a challenge, we suggest the establishment of a RVNT panel including other flaviviruses circulating in the region, associated with the other serological techniques such as IgM ELISA and the investigation of seroconversion, in order to help define an accurate diagnostic conclusion using serology.
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DOI：
10.1126/science.aaf5036
发表时间：
2016-04-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Faria NR;Azevedo RDSDS;Kraemer MUG;Souza R;Cunha MS;Hill SC;Thézé J;Bonsall MB;Bowden TA;Rissanen I;Rocco IM;Nogueira JS;Maeda AY;Vasami FGDS;Macedo FLL;Suzuki A;Rodrigues SG;Cruz ACR;Nunes BT;Medeiros DBA;Rodrigues DSG;Queiroz ALN;da Silva EVP;Henriques DF;da Rosa EST;de Oliveira CS;Martins LC;Vasconcelos HB;Casseb LMN;Simith DB;Messina JP;Abade L;Lourenço J;Alcantara LCJ;de Lima MM;Giovanetti M;Hay SI;de Oliveira RS;Lemos PDS;de Oliveira LF;de Lima CPS;da Silva SP;de Vasconcelos JM;Franco L;Cardoso JF;Vianez-Júnior JLDSG;Mir D;Bello G;Delatorre E;Khan K;Creatore M;Coelho GE;de Oliveira WK;Tesh R;Pybus OG;Nunes MRT;Vasconcelos PFC
通讯作者：
Vasconcelos PFC
影响因子：
4.6
作者：
Azevedo RSS;de Sousa JR;Araujo MTF;Martins Filho AJ;de Alcantara BN;Araujo FMC;Queiroz MGL;Cruz ACR;Vasconcelos BHB;Chiang JO;Martins LC;Casseb LMN;da Silva EV;Carvalho VL;Vasconcelos BCB;Rodrigues SG;Oliveira CS;Quaresma JAS;Vasconcelos PFC
通讯作者：
Vasconcelos PFC
影响因子：
8.8
作者：
Azevedo, Raimunda S. S.;Araujo, Marialva T.;Vasconcelos, Pedro F. C.
通讯作者：
Vasconcelos, Pedro F. C.
影响因子：
13.2
作者：
Calvez, Elodie;O'Connor, Olivia;Dupont-Rouzeyrol, Myrielle
通讯作者：
Dupont-Rouzeyrol, Myrielle
影响因子：
3.8
作者：
Haddow, Andrew D.;Nasar, Farooq;Weaver, Scott C.
通讯作者：
Weaver, Scott C.