Characterization of a novel gyrovirus in human stool and chicken meat.
Characterization of a novel gyrovirus in human stool and chicken meat.
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DOI：
10.1016/j.jcv.2012.07.001
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发表时间：
2012-11
影响因子：
8.8
通讯作者：
Peiris, J. S. Malik
中科院分区：
文献类型：
--
作者：
Chu, Daniel K. W.;Poon, Leo L. M.;Chiu, Susan S. S.;Chan, Kwok H.;Ng, Erica M.;Bauer, Irma;Cheung, Tim K.;Ng, Iris H. Y.;Guan, Yi;Wang, David;Peiris, J. S. Malik
临床标本的序列无关扩增可导致鉴定新的病原体。目的：鉴定腹泻患者粪便标本中存在的新型病毒，探讨该病毒的生态学及临床意义。从病因不明的腹泻患者粪便标本中提取核酸，进行随机PCR扩增和Roche/454焦磷酸测序。鉴定出的新型病毒具有遗传和流行病学特征。在人粪便标本中鉴定出鸡贫血病毒（CAV）、人陀螺病毒(HGV) /禽陀螺病毒2 （AGV2）、陀螺病毒3 （GyV3）和一种新型陀螺病毒（暂定为陀螺病毒4 (GyV4)）。在供人食用的鸡皮和肉中也检测到GyV4、CAV和AGV2/HGV。在人的粪便和供人食用的鸡肉中发现了一种新的回旋病毒（GyV4）。该病毒在系统发育上不同于以前报道的鸡和人的旋转病毒（鸡贫血病毒、人旋转病毒、禽旋转病毒2和最近报道的旋转病毒3）。该病毒在人类和鸡中的流行病学和发病机制有待进一步研究。
Sequence-independent amplification of clinical specimens can lead to the identification of novel pathogens. To identify novel viruses in human stool specimens from patients with diarrhea and to investigate the ecology and clinical significance of such viruses. Nucleic acid extracted from stool specimens from patients with diarrhea with no known aetiology were subjected to random PCR amplification and Roche/454 pyrosequencing. Novel viruses identified were genetically and epidemiologically characterized. Four gyroviruses, chicken anemia virus (CAV), human gyrovirus (HGV) / avian gyrovirus 2 (AGV2), gyrovirus 3 (GyV3) and a novel gyrovirus (tentatively designated as gyrovirus 4 (GyV4)) were identified in human stool specimens. GyV4, as well as CAV and AGV2/HGV were also detected in chicken skin and meat used for human consumption. A novel gyrovirus (GyV4) was identified in human stool and in chicken meat sold for human consumption. This virus was phylogenetically distinct from previously reported gyroviruses in chicken and humans (chicken anemia virus, human gyrovirus, avian gyrovirus 2 and recently reported gyrovirus 3). The epidemiology and pathogenesis of this virus in humans and in chicken needs to be further investigated.
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影响因子：
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作者：
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