Burn wound γδ T-cells support a Th2 and Th17 immune response.
Burn wound γδ T-cells support a Th2 and Th17 immune response.
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DOI：
10.1097/01.bcr.0000440705.91099.cc
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发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Schwacha MG
中科院分区：
文献类型：
--
作者：
Rani M;Zhang Q;Schwacha MG
严重烧伤引发免疫功能障碍，这与伤口愈合并发症有关。γ δ T细胞已被证明在烧伤后炎症和伤口愈合中是重要的;然而，它们在烧伤伤口部位的细胞因子表型是未知的。对C57 BL/6雄性小鼠进行严重烧伤（25% TBSA，3度）或假处理。在3小时、3天和7天后，收集皮肤样品并进行分散酶和胰蛋白酶消化以分离单细胞。对细胞进行表型分析，并通过流式细胞术评价细胞因子谱。Th-1细胞定义为IFNγ阳性，Th-2细胞定义为IL-10阳性，Th-17细胞定义为IL-17阳性。在烧伤后7天，观察到伤口部位的Th-2和Th-17阳性T细胞的转移。进一步分析显示，在损伤后3小时，假手术和烧伤皮肤样品之间IFNγ、IL-10和IL-17阳性的γδ T细胞的百分比相当。在损伤后3天和7天，对每种细胞因子呈阳性的细胞百分比增加;然而，与IFNγ相比，IL-10和IL-17的增加显著更大（即，9-20倍对3倍）。皮肤αβ T细胞优先产生IFNγ（~20%），这不受烧伤的影响。这些数据表明，烧伤伤口γδ Τ细胞被激活以增强细胞因子产生，并显示向Th-2和/或Th-17表型的转变。相比之下，烧伤伤口αβ T细胞未被激活以增强细胞因子产生。
Major burn triggers immune dysfunction, which is associated with wound healing complications. Gamma delta T-cells have been shown to be important in post-burn inflammation and wound healing; however their cytokine phenotype at the burn wound site is unknown. C57BL/6 male mice were subjected to a major burn (25% TBSA, 3rd degree) or sham treatment. At 3 h, 3 days and 7 days thereafter, skin samples were collected and subjected to dispase and trypsin digestion to isolate single cells. The cells were phenotyped and evaluated for cytokine profiles by flow cytometry. Th-1 cells were defined as IFNγ positive, Th-2 cells were defined IL-10 positive and Th-17 cells were defined as IL-17 positive. At 7 days after burn a shift towards Th-2 and Th-17 positive T-cells at the wound site was observed. Further analysis revealed that at 3 h post-injury the percentage of γδ T-cells positive for IFNγ, IL-10 and IL-17 where comparable between sham and burn skin samples. At 3 days and 7 days post-injury the percentage of cells positive for each cytokine increased; however, the increase was significantly greater for IL-10 and IL-17, as compared with IFNγ (i.e., 9-20 fold vs. 3-fold). Skin αβ T-cells preferentially produced IFNγ (~20%), which was unaffected by burn injury. These data demonstrate that burn wound γδ T-cells are activated for enhanced cytokine production and display a shift towards a Th-2 and/or Th-17 phenotype. In contrast, burn wound αβ T-cells were not activated for enhanced cytokine production.
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影响因子：
3.8
作者：
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通讯作者：
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影响因子：
100.3
作者：
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影响因子：
3.1
作者：
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通讯作者：
Schwacha, Martin G.
影响因子：
--
作者：
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通讯作者：
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