Retinoic acid upregulates ret and induces chain migration and population expansion in vagal neural crest cells to colonise the embryonic gut.
Retinoic acid upregulates ret and induces chain migration and population expansion in vagal neural crest cells to colonise the embryonic gut.
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DOI：
10.1371/journal.pone.0064077
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Newgreen DF
中科院分区：
文献类型：
--
作者：
Simkin JE;Zhang D;Rollo BN;Newgreen DF
迷走神经嵴细胞（VNCCs）产生于后脑，并在（禽类）胚胎日(E) 1.5时开始通过旁轴组织迁移，1-2天后作为细胞链到达前肠。然后，它们会在肠道的其他部分形成逆行波。迁移的细胞链随后进入肠神经系统的神经节。在器官培养中，居住在肠道（称为肠或ENCC）的E4.5 vnc先前遇到迷走旁轴组织，迅速以细胞链的形式大量定植于神经肠道组织。在同一时间段内，先前未暴露于旁轴组织的E1.5 vnc提供了很少的细胞进入肠道间质，并且这些细胞从未形成链，尽管它们能够在旁轴组织和常规细胞培养中迁移。将体外vnc暴露于通常在前往前肠途中遇到的旁轴组织中，可赋予其肠内迁移能力。VNCC通过旁轴组织后产生维甲酸信号元件，如维甲酸结合蛋白1的表达。通过将维甲酸或合成类维甲酸Am80添加到器官培养中的vnc（而不是主干nc）中，可以重现旁轴组织促进肠道定植的能力。维甲酸受体拮抗剂CD 2665可显著降低E1.5 VNCC和E4.5 ENCCs的肠道定植，其浓度提示RARα信号传导。经流式细胞术分析，维甲酸应用于迷走神经管和NCCs可上调Ret；一种由ENCCs表达的神经胶质来源的神经营养因子受体，是正常肠道定植所必需的。这表明早期VNCC虽然具有迁移性，但不能在肠间质中以适当的链迁移，但可以通过视黄酸启动。这是NCC迁移的特征形式的第一个实例，链式迁移，被归因于形态因子的应用。
Vagal neural crest cells (VNCCs) arise in the hindbrain, and at (avian) embryonic day (E) 1.5 commence migration through paraxial tissues to reach the foregut as chains of cells 1–2 days later. They then colonise the rest of the gut in a rostrocaudal wave. The chains of migrating cells later resolve into the ganglia of the enteric nervous system. In organ culture, E4.5 VNCCs resident in the gut (termed enteric or ENCC) which have previously encountered vagal paraxial tissues, rapidly colonised aneural gut tissue in large numbers as chains of cells. Within the same timeframe, E1.5 VNCCs not previously exposed to paraxial tissues provided very few cells that entered the gut mesenchyme, and these never formed chains, despite their ability to migrate in paraxial tissue and in conventional cell culture. Exposing VNCCs in vitro to paraxial tissue normally encountered en route to the foregut conferred enteric migratory ability. VNCC after passage through paraxial tissue developed elements of retinoic acid signalling such as Retinoic Acid Binding Protein 1 expression. The paraxial tissue's ability to promote gut colonisation was reproduced by the addition of retinoic acid, or the synthetic retinoid Am80, to VNCCs (but not to trunk NCCs) in organ culture. The retinoic acid receptor antagonist CD 2665 strongly reduced enteric colonisation by E1.5 VNCC and E4.5 ENCCs, at a concentration suggesting RARα signalling. By FACS analysis, retinoic acid application to vagal neural tube and NCCs in vitro upregulated Ret; a Glial-derived-neurotrophic-factor receptor expressed by ENCCs which is necessary for normal enteric colonisation. This shows that early VNCC, although migratory, are incapable of migrating in appropriate chains in gut mesenchyme, but can be primed for this by retinoic acid. This is the first instance of the characteristic form of NCC migration, chain migration, being attributed to the application of a morphogen.
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影响因子：
2.7
作者：
Desai, TJ;Malpel, S;Cardoso, WV
通讯作者：
Cardoso, WV
影响因子：
2.7
作者：
Broders-Bondon, Florence;Paul-Gilloteaux, Perrine;Dufour, Sylvie
通讯作者：
Dufour, Sylvie
影响因子：
2.5
作者：
Blentic, A;Gale, E;Maden, M
通讯作者：
Maden, M
DOI：
10.1007/s004290050150
发表时间：
1998-05-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
Broekhuizen, MLA;Gittenberger-de Groot, AC;Poelmann, RE
通讯作者：
Poelmann, RE
影响因子：
3.7
作者：
Diman, Nata Y. S. -G.;Remacle, Sophie;Rezsohazy, Rene
通讯作者：
Rezsohazy, Rene