High-resolution analytical electron microscopy reveals cell culture media-induced changes to the chemistry of silver nanowires.
High-resolution analytical electron microscopy reveals cell culture media-induced changes to the chemistry of silver nanowires.
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DOI：
10.1021/es403264d
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发表时间：
2013-12-03
影响因子：
11.4
通讯作者：
Porter, Alexandra E.
中科院分区：
文献类型：
--
作者：
Chen, Shu;Theodorou, Ioannis G.;Goode, Angela E.;Gow, Andrew;Schwander, Stephan;Zhang, Junfeng (Jim);Chung, Kian Fan;Tetley, Teresa D.;Shaffer, Milo S.;Ryan, Mary P.;Porter, Alexandra E.
人们越来越担心接触工程银纳米材料（颗粒、线和板）对人类健康可能产生的不利影响。然而，大多数测试银纳米材料毒性的研究都检查了名义上“合成时”的材料，而没有考虑材料在生物相关液体中的命运。在这里，通过改进的多元醇工艺制备了内部银纳米线 (AgNW)，并在三种细胞培养基（DMEM、RPMI-1640 和 DCCM-1）中孵育，以检查 AgNW-介质相互作用对 AgNW 理化性质的影响。高分辨率分析透射电子显微镜显示，在 DCCM-1 中孵育 1 小时内，Ag2S 晶体在 AgNW 表面形成。相反，AgNW 在 RPMI-1640 或 DMEM 中的孵育不会导致硫化。当DCCM-1细胞培养基分离为其小分子溶质和盐以及蛋白质组分时，发现AgNW在含有小分子溶质和盐的级分中硫化，但在含有培养基的蛋白质组分的级分中不硫化。进一步的研究表明，在存在分离的含硫氨基酸或蛋白质（例如半胱氨酸或牛血清白蛋白（BSA））的情况下，AgNW 不容易硫化。结果表明，AgNW 在与细胞孵育之前和期间可以被培养基转化，因此在毒性测定分析中必须考虑细胞培养基的影响。必须采取适当的介质和材料控制措施，以便准确预测此类商业相关纳米材料的毒性，并最终预测其健康风险。
There is a growing concern about the potential adverse effects on human health upon exposure to engineered silver nanomaterials (particles, wires and plates). However, the majority of studies testing the toxicity of silver nanomaterials have examined nominally ‘as-synthesized’ materials without considering the fate of the materials in biologically relevant fluids. Here, in-house silver nanowires (AgNWs) were prepared by a modified polyol process and were incubated in three cell culture media (DMEM, RPMI-1640 and DCCM-1) to examine the impact of AgNW-medium interactions on the physicochemical properties of the AgNWs. High-resolution analytical transmission electron microscopy revealed that Ag2S crystals form on the surface of AgNWs within 1 hour of incubation in DCCM-1. In contrast, the incubation of AgNWs in RPMI-1640 or DMEM did not lead to sulfidation. When the DCCM-1 cell culture medium was separated into its small molecule solutes and salts and protein components, the AgNWs were found to sulfidize in the fraction containing small molecule solutes and salts, but not in the fraction containing the protein component of the media. Further investigation showed the AgNWs did not readily sulfidize in the presence of isolated sulfur containing amino acids or proteins, such as cysteine or bovine serum albumin (BSA). The results demonstrate that the AgNWs can be transformed by the media before and during the incubation with cells and therefore the effects of cell culture media must be considered in the analysis of toxicity assays. Appropriate media and material controls must be in place to allow accurate predictions about the toxicity, and ultimately, the health risk of this commercially relevant class of nanomaterial.
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影响因子：
17.1
作者：
George S;Lin S;Ji Z;Thomas CR;Li L;Mecklenburg M;Meng H;Wang X;Zhang H;Xia T;Hohman JN;Lin S;Zink JI;Weiss PS;Nel AE
通讯作者：
Nel AE
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Dibrov, P;Dzioba, J;Häse, CC
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Häse, CC
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Choi, Okkyoung;Cleuenger, Thomas E.;Hu, Zhiqiang
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Hu, Zhiqiang
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2.9
作者：
Holt, KB;Bard, AJ
通讯作者：
Bard, AJ
影响因子：
3.5
作者：
Arora, S.;Jain, J.;Paknikar, K. M.
通讯作者：
Paknikar, K. M.