Characterization of a cell bridge variant connecting the nodose and superior cervical ganglia in the mouse: Prevalence, anatomical features, and practical implications.
Characterization of a cell bridge variant connecting the nodose and superior cervical ganglia in the mouse: Prevalence, anatomical features, and practical implications.
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DOI：
10.1002/cne.24936
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Gautron L
中科院分区：
文献类型：
--
作者：
Bookout AL;Gautron L
虽然自主神经节已在大鼠而非小鼠中进行了广泛研究，但人们对小鼠自主神经系统的解剖学重新产生了兴趣。本研究检查了一组 C57BL/6J 小鼠中连接颈部两个自主神经节（即结状神经节 (NG) 和颈上神经节 (SCG)）的细胞桥的患病率和解剖学特征。我们发现了 NG 和 SCG 颅极之间的细胞桥。该细胞桥呈管状，平均长度和宽度分别为 700 和 240 μm。细胞桥通常是单侧的，并且男性（38%）的神经节团中的细胞桥明显多于女性（21%）。在它的每个四肢上，都含有迷走神经传入神经和节后交感神经元的混合体。两个神经元群在细胞桥的中间突然相互替换。我们检查了有或没有细胞桥的 NG 样品中选定自主标记的 mRNA 表达。我们的结果表明，细胞桥富含节后交感神经元和迷走神经传入神经元的标记物。最后，使用 FluoroGold 显微注射到 NG 中，我们发现细胞桥的存在有时可能会导致 SCG 的无意污染。总之，本研究描述了由小鼠 NG 和 SCG 融合组成的细胞桥变体的解剖结构。我们的观察结果的实际意义是针对小鼠迷走神经传入的研究进行讨论的，这是一个越来越受欢迎的研究领域。这项研究描述了由小鼠结状神经节 (NG) 和颈上神经节 (SCG) 融合组成的细胞桥 (CB) 变体的解剖结构。这种吻合呈管状，在男性（38%）的神经节肿块中比女性（21%）更常见。它包含混合的迷走神经传入神经元（蓝色，TH-阳性，Hand2-和异凝集素B4阴性；紫色，TH-和Hand2-阴性，异凝集素B4阳性；灰色，TH-和Hand2-以及异凝集素B4阴性）和节后交感神经元（绿色，TH-和Hand2-阳性）。两个神经元群在细胞桥的中间突然相互替换。我们的观察结果的实际意义是针对小鼠迷走神经传入的研究进行讨论的，这是一个越来越受欢迎的研究领域。
While autonomic ganglia have been extensively studied in rats instead of mice, there is renewed interest in the anatomy of the mouse autonomic nervous system. This study examined the prevalence and anatomical features of a cell bridge linking two autonomic ganglia of the neck, namely, the nodose ganglion (NG) and the superior cervical ganglion (SCG) in a cohort of C57BL/6J mice. We identified a cell bridge between the NG and the cranial pole of the SCG. This cell bridge was tubular-shaped with an average length and width of 700 and 240 μm, respectively. The cell bridge was frequently unilateral and significantly more prevalent in the ganglionic masses from males (38%) than females (21%). On each of its extremities, it contained a mixed of vagal afferents and postganglionic sympathetic neurons. The two populations of neurons abruptly replaced each other in the middle of the cell bridge. We examined the mRNA expression for selected autonomic markers in samples of the NG with or without cell bridge. Our results indicated that the cell bridge was enriched in both markers of postganglionic sympathetic and vagal afferents neurons. Lastly, using FluoroGold microinjection into the NG, we found that the existence of a cell bridge may occasionally lead to the inadvertent contamination of the SCG. In summary, this study describes the anatomy of a cell bridge variant consisting of the fusion of the mouse NG and SCG. The practical implications of our observations are discussed with respect to studies of the mouse vagal afferents, an area of research of increasing popularity. This study describes the anatomy of a cell bridge (CB) variant consisting of the fusion of the mouse nodose ganglion (NG) and the superior cervical ganglion (SCG). This anastomosis was tubular-shaped and significantly more prevalent in the ganglionic masses from males (38%) than females (21%). It contained a mixed of vagal afferents (blue, TH-positive and Hand2- and isolectin B4-negative; purple, TH- and Hand2-negative and isolectin B4-positive; grey, TH- and Hand2- and isolectin B4-negative) and postganglionic sympathetic neurons (green, TH- and Hand2-positive). The two populations of neurons abruptly replaced each other in the middle of the cell bridge. The practical implications of our observations are discussed with respect to studies of the mouse vagal afferents, an area of research of increasing popularity.
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DOI：
10.1007/s10286-017-0478-7
发表时间：
2018-03
期刊：
Clinical autonomic research : official journal of the Clinical Autonomic Research Society
影响因子：
--
作者：
Espinosa-Medina I;Saha O;Boismoreau F;Brunet JF
通讯作者：
Brunet JF
影响因子：
2.7
作者：
Bratton, B. O.;Martelli, D.;McAllen, R. M.
通讯作者：
McAllen, R. M.
影响因子：
5.2
作者：
Fedele, Malena L. Mul;Galiana, Maria D.;Plano, Santiago A.
通讯作者：
Plano, Santiago A.
DOI：
10.1126/science.aat5236
发表时间：
2018-09-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kaelberer MM;Buchanan KL;Klein ME;Barth BB;Montoya MM;Shen X;Bohórquez DV
通讯作者：
Bohórquez DV
影响因子：
64.5
作者：
Chang RB;Strochlic DE;Williams EK;Umans BD;Liberles SD
通讯作者：
Liberles SD